Abstract：
OBJECTIVE: To assess the characteristics of blebs formed after Ahmed glaucoma valve (AGV) surgery in dogs using ultrasound biomicroscopy (UBM) and to analyze their correlation with postoperative intraocular pressure (IOP).
METHODS: 16 eyes (13 dogs) were diagnosed with primary angle-closure glaucoma and were followed up after AGV surgery from June 2021 to September 2023.
METHODS: In this prospective study, UBM examinations were performed to assess bleb characteristics, including bleb wall thickness and reflectivity. IOP at the time of UBM imaging and the duration from AGV surgery to UBM imaging were recorded. Histological examination of an enucleated eye removed due to uncontrolled IOP leading to blindness was also conducted.
RESULTS: A significant correlation was observed between IOP and relative reflectivity (Pearson r = 0.60; P = .01), and a negative correlation was observed between bleb wall thickness and relative reflectivity (Pearson r = -0.72; P = .002). No significant correlation was observed between the duration from AGV surgery to UBM imaging and either bleb wall thickness or relative reflectivity, respectively. Histological examination of the enucleated eye revealed collagen-rich fibrous encapsulation of the bleb wall, including myofibroblasts that exhibited positive α-smooth muscle actin immunostaining.
CONCLUSIONS: In dogs that underwent AGV surgery, less dense, thick-walled blebs on UBM tended to maintain IOP within the normal range. However, denser, thinner-walled blebs showed IOP levels above the normal range despite the use of antiglaucoma medications. UBM is a useful tool for evaluating bleb characteristics and their influence on IOP regulation after AGV surgery in dogs.
METHODS: 16 eyes (13 dogs) were diagnosed with primary angle-closure glaucoma and were followed up after AGV surgery from June 2021 to September 2023.
METHODS: In this prospective study, UBM examinations were performed to assess bleb characteristics, including bleb wall thickness and reflectivity. IOP at the time of UBM imaging and the duration from AGV surgery to UBM imaging were recorded. Histological examination of an enucleated eye removed due to uncontrolled IOP leading to blindness was also conducted.
RESULTS: A significant correlation was observed between IOP and relative reflectivity (Pearson r = 0.60; P = .01), and a negative correlation was observed between bleb wall thickness and relative reflectivity (Pearson r = -0.72; P = .002). No significant correlation was observed between the duration from AGV surgery to UBM imaging and either bleb wall thickness or relative reflectivity, respectively. Histological examination of the enucleated eye revealed collagen-rich fibrous encapsulation of the bleb wall, including myofibroblasts that exhibited positive α-smooth muscle actin immunostaining.
CONCLUSIONS: In dogs that underwent AGV surgery, less dense, thick-walled blebs on UBM tended to maintain IOP within the normal range. However, denser, thinner-walled blebs showed IOP levels above the normal range despite the use of antiglaucoma medications. UBM is a useful tool for evaluating bleb characteristics and their influence on IOP regulation after AGV surgery in dogs.
摘要：
目的：应用超声生物显微镜（UBM）评估犬Ahmed青光眼瓣膜（AGV）手术后形成的气泡特征，并分析其与术后眼压（IOP）的相关性。
方法：16只眼（13只狗）被诊断为原发性闭角型青光眼，并于2021年6月至2023年9月进行AGV手术后随访。
方法：在这项前瞻性研究中，进行UBM检查以评估气泡特征，包括气泡壁厚和反射率。记录UBM成像时的IOP以及从AGV手术到UBM成像的持续时间。还对由于不受控制的IOP导致失明而被摘除的眼球进行组织学检查。
结果：观察到IOP与相对反射率之间存在显着相关性（Pearsonr=0.60；P=0.01），气泡壁厚与相对反射率之间呈负相关（Pearsonr=-0.72；P=.002）。从AGV手术到UBM成像的持续时间与气泡壁厚或相对反射率之间没有观察到显着的相关性。分别。眼球摘除后的组织学检查显示，气泡壁富含胶原蛋白的纤维包裹，包括表现出阳性α-平滑肌肌动蛋白免疫染色的肌成纤维细胞。
结论：在接受AGV手术的狗中，密度较低，UBM上的厚壁气泡倾向于将IOP维持在正常范围内。然而,密度更大，尽管使用了抗青光眼药物,但薄壁气泡显示IOP水平高于正常范围.UBM是评估犬AGV手术后气泡特征及其对IOP调节影响的有用工具。
方法：16只眼（13只狗）被诊断为原发性闭角型青光眼，并于2021年6月至2023年9月进行AGV手术后随访。
方法：在这项前瞻性研究中，进行UBM检查以评估气泡特征，包括气泡壁厚和反射率。记录UBM成像时的IOP以及从AGV手术到UBM成像的持续时间。还对由于不受控制的IOP导致失明而被摘除的眼球进行组织学检查。
结果：观察到IOP与相对反射率之间存在显着相关性（Pearsonr=0.60；P=0.01），气泡壁厚与相对反射率之间呈负相关（Pearsonr=-0.72；P=.002）。从AGV手术到UBM成像的持续时间与气泡壁厚或相对反射率之间没有观察到显着的相关性。分别。眼球摘除后的组织学检查显示，气泡壁富含胶原蛋白的纤维包裹，包括表现出阳性α-平滑肌肌动蛋白免疫染色的肌成纤维细胞。
结论：在接受AGV手术的狗中，密度较低，UBM上的厚壁气泡倾向于将IOP维持在正常范围内。然而,密度更大，尽管使用了抗青光眼药物,但薄壁气泡显示IOP水平高于正常范围.UBM是评估犬AGV手术后气泡特征及其对IOP调节影响的有用工具。
