Abstract：
In this study, we developed a process combining dilute alkali (NaOH or NaHCO3) and physical (disk milling and/or ball milling) treatments to improve the functionality and fermentability of corn fiber. The results showed that combining chemical with physical processes greatly improved the functionality and fermentability of corn fiber. Corn fiber treated with NaOH followed by disk milling (NaOH-DM-CF) had the highest water retention (19.5 g/g), water swelling (38.8 mL/g), and oil holding (15.5 g/g) capacities. Moreover, NaOH-DM-CF produced the largest amount (42.9 mM) of short-chain fatty acid (SCFA) during the 24-hr in vitro fermentation using porcine fecal inoculum. In addition, in vitro fermentation of NaOH-DM-CF led to a targeted microbial shifting to Prevotella (genus level), aligning with a higher fraction of propionic acid. The outstanding functionality and fermentability of NaOH-DM-CF were attributed to its thin and loose structure, decreased ester linkages and acetyl groups, and enriched structural carbohydrate exposure.
摘要：
在这项研究中,我们开发了一种将稀碱（NaOH或NaHCO3）和物理（圆盘研磨和/或球磨）处理相结合的方法，以改善玉米纤维的功能和可发酵性。结果表明，化学和物理过程的结合大大提高了玉米纤维的功能性和发酵性。用NaOH处理，然后进行圆盘研磨（NaOH-DM-CF）的玉米纤维具有最高的保水性（19.5g/g），水溶胀（38.8mL/g），和持油（15.5克/克）的能力。此外，NaOH-DM-CF在使用猪粪便接种物的24小时体外发酵过程中产生最大量（42.9mM）的短链脂肪酸（SCFA）。此外,NaOH-DM-CF的体外发酵导致靶向微生物转移到普雷沃氏菌(属水平)，与较高比例的丙酸对齐。NaOH-DM-CF突出的功能性和可发酵性归因于其薄而松散的结构，酯键和乙酰基减少，和丰富的结构性碳水化合物暴露。
