PDF(Pubmed)
Abstract：
Terpenes are important mediators of plant chemical response to environmental cues. Here, we describe the genome-wide identification and biochemical characterization of TPS-a members in Medicago truncatula, a model legume crop. Genome mining identified thirty-nine full-length terpene synthases with a significant number predicted to produce monoterpenes and sesquiterpenes. Biochemical characterization of the TPS-a subfamily associated with sesquiterpene biosynthesis revealed such compounds, that exhibit substantial biological activity in other plants. Gene expression analysis using qPCR and the Medicago gene atlas illustrated distinct tissue and time-based variation in expression in leaves and roots. Together our work establishes the gene-to-metabolite relationships for sesquiterpene synthases in M. truncatula. Understanding the biosynthetic capacity is a foundational step to defining the ecological roles of this important family of compounds.
摘要：
萜烯是植物对环境线索的化学反应的重要介质。这里,我们描述了Medicagotruncatula中TPS-a成员的全基因组鉴定和生化表征，豆科植物模型。基因组挖掘确定了39种全长萜烯合酶，其中有大量预测会产生单萜和倍半萜。与倍半萜生物合成相关的TPS-a亚家族的生化表征揭示了此类化合物，在其他植物中表现出显著的生物活性。使用qPCR和Medicago基因图谱的基因表达分析显示了叶片和根中表达的不同组织和基于时间的变化。我们的工作共同建立了M.truncatula中倍半萜合酶的基因与代谢物关系。了解生物合成能力是定义这一重要化合物家族的生态作用的基础步骤。
