Abstract：
This study explores Neutrophil Extracellular Trap (NET) formation in equine neutrophils, which is crucial for eliminating infections and is implicated in various equine inflammatory diseases. We investigated the molecular pathways involved in NET release by equine neutrophils in response to stimuli. We use PMA, A23187, LPS, PAF, OZ, and cytokines, observing NET release in response to PMA, PAF, and A23187. In contrast, LPS, OZ, and the cytokines tested did not induce DNA release or did not consistently induce citrullination of histone 4. Peptidyl-arginine deiminase inhibition completely halted NET release, while NADPH oxidase and mitochondrial reactive oxygen species only played a role in PMA-induced NETs. Neutrophil elastase inhibition modestly affected PAF-induced NET liberation but not in PMA or A23187-induced NET, while myeloperoxidase did not contribute to NET release. We expect to provide a foundation for future investigations into the role of NETs in equine health and disease and the search for potential therapeutic targets.
摘要：
本研究探讨了马中性粒细胞中中性粒细胞胞外捕获（NET）的形成，这对于消除感染至关重要，并且与各种马炎性疾病有关。我们研究了马中性粒细胞响应刺激而释放NET的分子途径。我们用PMA,A23187,LPS,PAF,OZ,和细胞因子，观察NET释放以响应PMA，PAF,A23187相比之下,LPS,OZ,并且所测试的细胞因子不诱导DNA释放或不一致地诱导组蛋白4的瓜氨酸化。肽基精氨酸脱亚胺酶抑制完全停止NET释放，而NADPH氧化酶和线粒体活性氧仅在PMA诱导的NETs中起作用。中性粒细胞弹性蛋白酶抑制适度影响PAF诱导的NET释放，但不影响PMA或A23187诱导的NET，而髓过氧化物酶对NET的释放没有贡献。我们希望为未来研究NETs在马健康和疾病中的作用以及寻找潜在的治疗靶标提供基础。
