Abstract：
Ischemia/reperfusion (I/R) injury of sciatic nerve is a serious condition that results in nerve fiber degeneration, and reperfusion causes oxidative injury. Peripheral blood mononuclear cells (PBMNCs) have neuroregenerative power. This study was carried out to evaluate the potential ameliorative effect of PBMNCs on changes induced by I/R injury of the sciatic nerve. Fifty adult male albino rats were divided into donor and experimental groups that were subdivided into four groups: group I (control group), group II received 50 µL PBNMCs once intravenously via the tail vein, group III rubber tourniquet was placed around their Rt hind limb root for 2 hours to cause ischemia, group IV was subjected to limb ischemia as group III, then they were injected with 50 ul PBMNCs as group II before reperfusion. I/R injury showed disorganization of nerve fascicles with wide spaces in between nerve fibers. The mean area of collagen fibers, iNOS immunoexpression, and number of GFAP-positive Schwann cells of myelinated fibers showed a highly significant increase, while a highly significant reduction in the G-ratio and neurofilament immunoexpression was observed. Myelin splitting, invagination, evagination, and myelin figures were detected. PBMNC-treated group showed a marked improvement that was confirmed by histological, immunohistochemical, and ultrastructural findings.
摘要：
坐骨神经缺血/再灌注(I/R)损伤是导致神经纤维变性的严重疾病,再灌注引起氧化损伤。外周血单核细胞(PBMNCs)具有神经再生能力。这项研究旨在评估PBMNCs对坐骨神经I/R损伤引起的变化的潜在改善作用。将50只成年雄性白化病大鼠分为供体组和实验组，再分为四组：I组（对照组），II组经尾静脉一次接受50微升PBNMC,第三组橡胶止血带放置在他们的Rt后肢根周围2小时，以引起缺血，IV组作为III组进行肢体缺血，然后在再灌注前注射50μlPBMNCs作为第II组。I/R损伤表现为神经束的紊乱，神经纤维之间有很宽的空间。胶原纤维的平均面积，iNOS免疫表达，GFAP阳性的有髓纤维雪旺细胞数量显著增加，同时观察到G比率和神经丝免疫表达的显着降低。髓鞘分裂，内陷，逃避,和髓鞘的数字被检测到。PBMNC治疗组显示出明显的改善，通过组织学证实，免疫组织化学，和超微结构的发现。
