Abstract：
Background: Trypanosoma (T.) evansi infection is endemic in dromedary camels (Camelus dromedaries) of southern Algeria. Materials and Methods: In order to assess the presence of T. evansi in other domestic animals living together with dromedary camels, a study was conducted in the wilayate of Béchar, El Bayadh, Ouargla and Tamanrasset, between 2015 and 2017. Authorisation to conduct the survey was obtained from the Direction des Services Vétérinaires (DSV, Ministry of Agriculture, Rural Development and Fisheries). A total of 190 animals were sampled, including 42 cattle (Bos taurus), 11 dogs (Canis familiaris), 44 horses (Equus caballus), 3 donkeys (Equus asinus) and 1 mule, 49 goats (Capra hircus) and 40 sheep (Ovis aries). These animals were examined by parasitological (Giemsa stained thin smear, GST), serological (card agglutination test for trypanosomosis (CATT/T. evansi), enzyme-linked immunosorbent assay/Variant Surface Glycoprotein/Rode Trypanozoon antigen type 1.2 [ELISA/VSG RoTat 1.2], immune trypanolysis [TL]) and molecular tests (T. evansi type A specific RoTat 1.2 PCR). Results and Conclusions: The CATT/T. evansi was positive in 10/42 cattle, 0/11 dogs, 2/48 equids, 27/49 goats and 15/40 sheep. On the other hand, 20/38 cattle, 1/9 dogs, 21/42 equids, 17/44 goats and 31/39 sheep were positive in ELISA/VSG RoTat 1.2. However, no single animal was positive in TL. In addition, the T. evansi parasite could not be demonstrated by either GST or RoTat 1.2 PCR in any of the examined animals. This may suggest cross-reactions of CATT/T. evansi and ELISA/VSG RoTat 1.2 with other pathogenic or commensal trypanosome species such as T. vivax or other parasites. Based on these data, in particular taking into account the high specificity of the TL for T. evansi type A, this study does not support the hypothesis that T. evansi circulates in the studied domestic animal species and that they would act as reservoirs for the parasite that causes trypanosomosis in dromedary camels.
摘要：
背景：锥虫（T.）伊凡斯人感染在阿尔及利亚南部的单峰骆驼（骆驼单峰骆驼）中流行。材料和方法：为了评估与单峰骆驼一起生活的其他家养动物中伊万西氏菌的存在，在Béchar的田地进行了一项研究，ElBayadh,Ouargla和Tamanrasset,2015年至2017年。进行调查的授权是从服务部门获得的(DSV，农业部,农村发展和渔业)。总共采集了190只动物的样本，包括42头牛(Bostaurus)，11只狗（犬），44匹马（Equuscaballus），3头驴（马）和1头骡子，49只山羊（Caprahircus）和40只绵羊（Ovisaries）。通过寄生虫学检查这些动物（Giemsa染色的薄涂片，商品及服务税),血清学(锥虫病卡凝集试验(CATT/T.evansi),酶联免疫吸附测定/变体表面糖蛋白/Rode锥虫抗原1.2型[ELISA/VSGRoTat1.2]，免疫锥虫分解[TL])和分子测试(T.evansiA型特异性RoTat1.2PCR)。结果与结论：CATT/T。Evansi在10/42头牛中呈阳性，0/11狗2/48当量,27/49山羊和15/40绵羊。另一方面,20/38头牛,1/9狗21/42等同，17/44山羊和31/39绵羊在ELISA/VSG中RoTat为阳性1.2。然而,无单个动物TL阳性。此外,在任何被检查的动物中，GST或RoTat1.2PCR均无法证明伊万氏疟原虫。这可能表明CATT/T的交叉反应。evansi和ELISA/VSGRoTat1.2与其他致病性或共生锥虫物种，如间日疟原虫或其他寄生虫。基于这些数据,特别是考虑到TL对T.evansiA型的高特异性，这项研究不支持以下假设：T.evansi在所研究的家畜物种中循环，并且它们将充当导致单峰骆驼锥虫病的寄生虫的水库。
