PDF(Pubmed)
Abstract：
Canine bufavirus (CBuV) was reported in domestic dogs worldwide. We conducted a survey of canine bufavirus in domestic dogs in Thailand from September 2016 to October 2022. Rectal swab samples (n = 531) were collected from asymptomatic dogs and dogs with gastroenteritis signs. The samples were tested for CBuV using PCR with specific primers to the VP1/VP2 gene, and 9.42% (50/531) was CBuV positive. Our findings showed that CBuVs could be detected in both symptomatic and healthy dogs. The Thai CBuVs were found in dogs from different age groups, with a significant presence in those under 1 year (12.60%) and dogs aged 1-5 years (7.34%) (p < 0.05), suggesting a high prevalence of Thai CBuVs in dogs under 5 years of age. We performed complete genome sequencing (n = 15) and partial VP1/VP2 sequencing (n = 5) of Thai CBuVs. Genetic and phylogenetic analyses showed that whole genomes of Thai CBuVs were closely related to Chinese and Italian CBuVs, suggesting the possible origin of Thai CBuVs. The analysis of VP1 and VP2 genes in Thai CBuVs showed that 18 of them were placed in subgroup A, while only 2 belonged to subgroup B. This study is the first to report the detection and genetic characterization of CBuVs in domestic dogs in Thailand. Additionally, surveillance and genetic characterization of CBuVs in domestic animals should be further investigated on a larger scale to elucidate the dynamic, evolution, and distribution of CBuVs.
摘要：
在全世界的家犬中报道了犬科病毒(CBuV)。我们于2016年9月至2022年10月在泰国的家犬中进行了犬布法病毒的调查。从无症状狗和具有胃肠炎体征的狗收集直肠拭子样品(n=531)。使用具有针对VP1/VP2基因的特异性引物的PCR测试样品的CBuV,CBuV阳性9.42%(50/531)。我们的发现表明，在有症状和健康的狗中都可以检测到CBuV。泰国CBuV在不同年龄段的狗中发现，在1岁以下（12.60％）和1-5岁（7.34％）的狗中存在显着存在（p<0.05），提示5岁以下狗的泰国CBuV患病率很高。我们对泰国CBuV进行了完全基因组测序(n=15)和部分VP1/VP2测序(n=5)。遗传和系统发育分析表明，泰国CBUV的全基因组与中国和意大利CBUV密切相关，表明泰国CBUV的可能起源。对泰国CBuV中VP1和VP2基因的分析显示，其中18个被置于A亚组，而只有2个属于B亚组。这项研究是首次报道泰国家犬中CBUV的检测和遗传表征。此外,监测和遗传特征的cbuv在家养动物应进一步调查在更大范围内阐明的动态，进化,和CBUV的分布。
