PDF(Pubmed)
Abstract：
Ifosfamide (IFO), an alkylating chemotherapy agent, is known for its association with neurotoxicity and encephalopathy. This trial was designed to evaluate the protective action of daidzein (DZN) against IFO-induced neurotoxicity in male rats by determining the difference in certain inflammatory and apoptotic markers in the brain tissue of rats. Twenty-eight Wistar rats, weighing 120-150 g, were divided into four groups of seven rats: Group 1 (Control) received no treatment; Group 2 was orally administered DZN (100 mg/kg/day) for seven days; Group 3 received a single intraperitoneal (IP) dose of IFO (500 mg/kg); Group 4 received oral DZN (100 mg/kg/day) for one week prior to a single IP dose of IFO on the seventh day. Twenty-four hours post-treatment, serum and brain tissue samples were collected for analysis. The results indicated a significant increase in serum inflammatory markers (TNF-alpha, IL-6, and iNOS) and the anti-inflammatory marker (IL-10), along with elevated caspase-3 enzyme activity in the brain tissue of the IFO-treated group compared to the control group. Conversely, pre-treatment with DZN significantly reduced serum inflammatory markers and caspase-3 levels in tissue. The findings suggest that daidzein has anti-inflammatory and anti-apoptotic properties, potentially offering protection against IFO-induced neurotoxicity in rats.
摘要：
异环磷酰胺(IFO),烷化疗剂，以其与神经毒性和脑病的关联而闻名。该试验旨在通过确定大鼠脑组织中某些炎症和凋亡标志物的差异来评估大豆苷元（DZN）对IFO诱导的雄性大鼠神经毒性的保护作用。二十八只Wistar老鼠,重量120-150克，分为四组，每组7只大鼠：第1组（对照组）不接受治疗；第2组口服DZN（100mg/kg/天）7天；第3组接受单次腹膜内（IP）剂量的IFO（500mg/kg）；第4组接受口服DZN（100mg/kg/天），然后在第七天接受单次IP剂量的IFO。治疗后24小时，收集血清和脑组织样本进行分析.结果表明血清炎症标志物（TNF-α，IL-6和iNOS）和抗炎标志物（IL-10），与对照组相比，IFO处理组的脑组织中caspase-3酶活性升高。相反，用DZN预处理显著降低血清炎症标志物和组织中的caspase-3水平。研究结果表明，大豆苷元具有抗炎和抗凋亡特性，可能提供对IFO诱导的大鼠神经毒性的保护。
