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Abstract：
In individuals with type 2 diabetes mellitus (T2DM), the cannabinoid receptor 1 (CNR1) gene polymorphism has been linked to diabetic nephropathy (DN). Different renal disorders, including DN, have been found to alter cannabinoid (CB) receptor expression and activation. This cross-sectional study aimed to investigate the relationship between CNR1 rs1776966256 and rs1243008337 genetic variants and the risk of developing DN in Iraqi patients with T2DM. The study included 100 patients with T2DM, divided into two groups: 50 with DN and 50 without DN. Genotyping of CNR1 rs1776966256 and rs1243008337 polymorphisms was conducted using PCR in DN patients and control samples. The distribution of rs1776966256 and rs1243008337 genotypes and alleles between the two groups revealed statistically significant differences. The frequencies of the GG and AG genotypes of CNR1 rs1776966256 were significantly different between DN patients and the control group. Additionally, compared to the A allele, the G allele of this polymorphism was linked to a higher incidence of DN (p=0.0001). Patients with the genetic polymorphism rs1243008337 had higher genotypes of CC and AC and were more likely to develop DN in the polymorphism genotype than the wild genotype. Additionally, compared to the A allele, the C allele was linked to a higher chance of developing DN (p=0.0001). Both rs1776966256 and rs1243008337 polymorphisms were correlated with the development of diabetic nephropathy.
摘要：
在2型糖尿病（T2DM）患者中，大麻素受体1（CNR1）基因多态性与糖尿病肾病（DN）相关。不同的肾脏疾病，包括DN，已经发现改变大麻素(CB)受体的表达和激活。这项横断面研究旨在调查CNR1rs1776966256和rs1243008337遗传变异与伊拉克T2DM患者发生DN的风险之间的关系。该研究包括100名T2DM患者，分为两组：50个DN和50个无DN。在DN患者和对照样品中使用PCR进行CNR1rs1776966256和rs1243008337多态性的基因分型。rs1776966256和rs1243008337基因型和等位基因在两组之间的分布显示出统计学上的显着差异。CNR1rs1776966256的GG和AG基因型频率在DN患者和对照组之间存在显着差异。此外,与A等位基因相比，该多态性的G等位基因与DN的较高发病率相关(p=0.0001).具有基因多态性rs1243008337的患者具有较高的CC和AC基因型，并且在多态性基因型中比野生型基因型更容易发展为DN。此外,与A等位基因相比，C等位基因与较高的发展DN的机会相关（p=0.0001）。rs1776966256和rs1243008337多态性均与糖尿病肾病的发生发展相关。
