PDF(Pubmed)
Abstract：
Natural deep eutectic solvents (NADESs) can serve as solvents for enzymes, are biodegradable, and have low toxicities. Eight NADESs with different hydrogen bond acceptors and donors were tested to improve the stability and activity of a lipoxygenase from Basidiomycete Pleurotus sapidus (LOXPSA). Betaine:sorbitol:water (1:1:3, BSorbW) and betaine:ethylene glycol (1:3, BEtGly) had the best impact on the peroxidation of linoleic acid and the side reaction of piperine to the vanilla-like scented compound piperonal. The yield of piperonal in NADESs increased by 43% in BSorbW and 40% in BEtGly compared to the control. The addition of BSorbW also enhanced the enzyme\'s stability at various temperatures and increased its activity during incubation at 60 °C. The demonstrated improvement in lipoxygenase activity and stability indicates versatile applications in industry, expanding the potential uses of the enzyme.
摘要：
天然低共熔溶剂（NADES）可以作为酶的溶剂，是可生物降解的，毒性低。测试了八种具有不同氢键受体和供体的NADES，以提高侧耳担子菌（LOXPSA）的脂加氧酶的稳定性和活性。甜菜碱：山梨糖醇：水（1：1：3，BSorbW）和甜菜碱：乙二醇（1：3，BEtGly）对亚油酸的过氧化作用以及胡椒碱与香草味化合物胡椒醛的副反应具有最佳影响。与对照相比，NADESs中胡椒醛的产率在BSorbW中增加了43％，在BEtGly中增加了40％。BSorbW的添加还增强了酶在各种温度下的稳定性，并在60°C孵育期间增加了其活性。脂加氧酶活性和稳定性的改善表明在工业中具有广泛的应用，扩大酶的潜在用途。
