PDF(Pubmed)
Abstract：
Melatonin, a pleiotropic small molecule, is employed in horticultural crops to delay senescence and preserve postharvest quality. In this study, 100 µM melatonin treatment delayed a decline in the color difference index h* and a*, maintaining the content of chlorophyll and carotenoids, thereby delaying the yellowing and senescence of Chinese kale. Transcriptome analysis unequivocally validates melatonin\'s efficacy in delaying leaf senescence in postharvest Chinese kale stored at 20 °C. Following a three-day storage period, the melatonin treatment group exhibited 1637 differentially expressed genes (DEGs) compared to the control group. DEG analysis elucidated that melatonin-induced antisenescence primarily governs phenylpropanoid biosynthesis, lipid metabolism, plant signal transduction, and calcium signal transduction. Melatonin treatment up-regulated core enzyme genes associated with general phenylpropanoid biosynthesis, flavonoid biosynthesis, and the α-linolenic acid biosynthesis pathway. It influenced the redirection of lignin metabolic flux, suppressed jasmonic acid and abscisic acid signal transduction, and concurrently stimulated auxin signal transduction. Additionally, melatonin treatment down-regulated RBOH expression and up-regulated genes encoding CaM, thereby influencing calcium signal transduction. This study underscores melatonin as a promising approach for delaying leaf senescence and provides insights into the mechanism of melatonin-mediated antisenescence in postharvest Chinese kale.
摘要：
褪黑激素,多效性小分子，用于园艺作物以延缓衰老并保持采后质量。在这项研究中,100µM褪黑素治疗延迟了色差指数h*和a*的下降，保持叶绿素和类胡萝卜素的含量，从而延缓了羽衣甘蓝的黄化和衰老。转录组分析明确验证了褪黑激素在20°C贮藏的羽衣甘蓝中延缓叶片衰老的功效。经过三天的储存期，与对照组相比,褪黑素治疗组表现出1637个差异表达基因(DEGs).DEG分析阐明褪黑激素诱导的抗衰老主要控制苯丙素生物合成，脂质代谢，植物信号转导，和钙信号转导。褪黑素处理上调与一般苯丙素生物合成相关的核心酶基因，类黄酮生物合成，和α-亚麻酸生物合成途径。它影响了木质素代谢通量的重定向，抑制茉莉酸和脱落酸信号转导，同时刺激生长素信号转导。此外,褪黑素治疗下调RBOH表达和上调编码CaM的基因，从而影响钙信号转导。这项研究强调了褪黑激素是延缓叶片衰老的有希望的方法，并提供了对采后中国羽衣甘蓝中褪黑激素介导的抗衰老机制的见解。
