Abstract：
Cell division is a conserved process among eukaryotes. It is designed to segregate chromosomes into future daughter cells and involves a complex rearrangement of the cytoskeleton, including microtubules and actin filaments. An additional level of complexity is present in asymmetric dividing stem cells because cytoskeleton elements are also regulated by polarity cues. The neural stem cell system of the fruit fly represents a simple model to dissect the mechanisms that control cytoskeleton reorganization during asymmetric division. In this chapter, we propose to describe protocols that allow accurate analysis of microtubule reorganization during cell division in this model.
摘要：
细胞分裂是真核生物之间的保守过程。它旨在将染色体分离为未来的子细胞，并涉及细胞骨架的复杂重排，包括微管和肌动蛋白丝。不对称分裂干细胞中存在额外的复杂性水平，因为细胞骨架元件也受极性线索调节。果蝇的神经干细胞系统代表了一个简单的模型，可以解剖不对称分裂过程中控制细胞骨架重组的机制。在这一章中,我们建议描述允许在该模型中的细胞分裂过程中对微管重组进行准确分析的协议。
