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Abstract：
In this study, 15 Lulworthiales strains isolated from the marine tunicate Halocynthia papillosa collected in the central Tyrrhenian Sea were characterized using a polyphasic approach (morpho-physiological, molecular, and phylogenetic analyses). Based on multi-locus phylogenetic inference and morphological characters, a new genus, Rambellisea, and two new species, R. halocynthiae and R. gigliensis (Lulworthiales), were proposed. Multi-locus phylogenetic analyses using the nuclear ribosomal regions of DNA (nrITS1-nr5.8S-nrITS2, nrLSU, and nrSSU) sequence data strongly supported the new taxa. Phylogenetic inference, estimated using Maximum Likelihood and Bayesian Inference, clearly indicates that Rambellisea gen. nov. forms a distinct clade within the order Lulworthiales. Moreover, the two new species were separated into distinct subclades, solidly supported by the analyses. This is the first report of Lulworthiales species isolated from animals.
摘要：
在这项研究中,使用多相方法对从Tyrenian海中部收集的海洋被膜Halocynthiapapillosa中分离出的15个Lulworthiales菌株进行了表征（形态生理，分子，和系统发育分析)。基于多基因座系统发育推断和形态特征,一个新的属，Rambellisea,和两个新物种，卤虫和吉格里纳斯(Lulworthales)，被提议。使用DNA的核核糖体区域进行多位点系统发育分析（nrITS1-nr5.8S-nrITS2，nrLSU，和nrSSU)序列数据强烈支持新分类群。系统发育推断，使用最大似然和贝叶斯推断进行估计，清楚地表明Rambelliseagen。11月。在Lulworthiales命令中形成了一个独特的进化枝。此外，这两个新物种被分成不同的子分支，得到分析的有力支持。这是从动物中分离出的Lulworthiales物种的第一份报告。
