真菌的正确分类,包括酵母,了解真菌生物多样性和交流这种多样性至关重要。真菌属的定义主要基于表型特征和基于单基因或多基因的系统发育分析的结果。然而,因为酵母通常具有较少的表型特征,他们的分类经历了向基于DNA的数据的强有力的转变,从短核糖体序列到多基因系统发育,以及最近的系统发育。这里,我们以酵母结构域为例,探讨了各种基于基因组学的参数对更正确地界定真菌属的有用性。因此,我们比较了系统发育分析的结果,平均氨基酸同一性(AAI)值,保守签名索引(CSI)的存在,保守蛋白(POCP)的百分比和直系同源物(PAPO)的存在-不存在模式。这些基于基因组的指标用于研究它们在划分13个迄今相对被接受的酵母菌科属中的有用性,即Eremothecium,Grigorovia,Kazachstania,克鲁维酵母,Lachancea,Nakaseomyces,Naumovozyma,酵母菌,四角孢菌,Torulaspora,Vanderwaltozyma,酵母菌和Zygotorulaspora。因此,这些属中的大多数都得到了基于基因组学的指标的支持,但是Kazachstania属,根据上述分析,Nakaseomyces和Tetrapidisspora在遗传上具有高度多样性。考虑到目前公认的属获得的结果,80-92%的POCP值范围和60-70%的AAI值范围可能是区分酵母菌科属的有价值的阈值。此外,发现在PAPO分析和CSIs中鉴定出的属特异性基因可用作突触体,以表征和定义酵母菌科中的属。我们的结果表明,基于单生的系统基因组分析与基因组相关性指数和突触组织相结合,为描绘酵母属和丝状真菌的属提供了有希望的方法。Kazachstania属,对Nakaseomyces和Tetrapipisspora进行了修订,我们提出了8个新属和41个新组合。引文:刘f,胡Z-D,YurkovA,etal.2024.酵母菌科:基于系统发育分析的真菌属的分类,基因组相关性指数和基于基因组学的突触。Persoonia52:1-21。https://doi.org/10.3767/persoonia.2024.52.01。
A correct classification of fungi, including yeasts, is of prime importance to understand fungal biodiversity and to communicate about this diversity. Fungal genera are mainly defined based on phenotypic characteristics and the results of single or multigene-based phylogenetic analyses. However, because yeasts often have less phenotypic characters, their classification experienced a strong move towards DNA-based data, from short ribosomal sequences to multigene phylogenies and more recently to phylogenomics. Here, we explore the usefulness of various genomics-based parameters to circumscribe fungal genera more correctly taking the yeast domain as an example. Therefore, we compared the results of a phylogenomic analysis, average amino acid identity (AAI) values, the presence of conserved signature indels (CSIs), the percentage of conserved proteins (POCP) and the presence-absence patterns of orthologs (PAPO). These genome-based metrics were used to investigate their usefulness in demarcating 13 hitherto relatively well accepted genera in Saccharomycetaceae, namely Eremothecium, Grigorovia, Kazachstania, Kluyveromyces, Lachancea, Nakaseomyces, Naumovozyma, Saccharomyces, Tetrapisispora, Torulaspora, Vanderwaltozyma, Zygosaccharomyces and Zygotorulaspora. As a result, most of these genera are supported by the genomics-based metrics, but the genera Kazachstania, Nakaseomyces and Tetrapisispora were shown to be genetically highly diverse based on the above listed analyses. Considering the results obtained for the presently recognized genera, a range of 80-92 % POCP values and a range of 60-70 % AAI values might be valuable thresholds to discriminate genera in Saccharomycetaceae. Furthermore, the genus-specific genes identified in the PAPO analysis and the CSIs were found to be useful as synapomorphies to characterize and define genera in Saccharomycetaceae. Our results indicate that the combined monophyly-based phylogenomic analysis together with genomic relatedness indices and synapomorphies provide promising approaches to delineating yeast genera and likely those of filamentous fungi as well. The genera Kazachstania, Nakaseomyces and Tetrapisispora are revised and we propose eight new genera and 41 new combinations. Citation: Liu F, Hu Z-D, Yurkov A, et al. 2024. Saccharomycetaceae: delinaeation of fungal genera based on phylogenomic analyses, genomic relatedness indices and genomics-based synapomorphies. Persoonia 52: 1-21. https://doi.org/10.3767/persoonia.2024.52.01.