Abstract：
Fluorophores bearing cationic pendants, such as the pyridinium group, tend to preferentially accumulate in mitochondria, whereas those with pentafluorophenyl groups display a distinct affinity for the endoplasmic reticulum. In this study, we designed fluorophores incorporating pyridinium and pentafluorophenyl pendants and examined their impact on sub-cellular localization. Remarkably, the fluorophores exhibited a notable propensity for the mitochondrial membrane. Furthermore, these fluorophores revealed dual functionality by facilitating the detection of viscosity changes within the sub-cellular environment and serving as heavy-atom-free photosensitizers. With easy chemical tunability, wash-free imaging, and a favorable signal-to-noise ratio, these fluorophores are valuable tools for imaging mitochondria and investigating their cellular processes.
摘要：
含阳离子吊坠的荧光团,例如吡啶鎓基团，倾向于优先在线粒体中积累，而具有五氟苯基的那些对内质网表现出明显的亲和力。在这项研究中,我们设计了包含吡啶鎓和五氟苯基侧基的荧光团，并检查了它们对亚细胞定位的影响。值得注意的是,荧光团表现出明显的线粒体膜倾向。此外，这些荧光团通过促进检测亚细胞环境中的粘度变化并充当无重原子的光敏剂而显示出双重功能。具有容易的化学可调性，免清洗成像，和良好的信噪比，这些荧光团是线粒体成像和研究其细胞过程的有价值的工具。
