Abstract：
Since its introduction as a model organism in the 1980s, the use of zebrafish (Danio rerio) in research has expanded worldwide. Despite its now widespread use in research, guidelines to safeguard the ethical treatment of zebrafish, particularly with regard to euthanasia and humane endpoint practices, remain inadequate. One well-recognized example is the use of excess tricaine methanesulfonate (MS-222) as a means to euthanize zebrafish, regardless of life stage. In this study, through nationwide expert elicitation, we provide a detailed account of zebrafish research practices within the Republic of Korea and the challenges of implementing appropriate methods for euthanasia as a humane endpoint, with many opting for hypothermic shock. We report a local expert consensus for establishing national guidelines to improve zebrafish welfare and good research practice. Suggestions and recommendations for national guidelines were offered. Taken together, our findings raise awareness broadly among zebrafish research practitioners in the field, offer an accurate account of the welfare and treatment of zebrafish in research within the Republic of Korea, and advocate for the development and implementation of national guidelines. As such, our study is useful as a model to adopt the expert elicitation approach to investigate, quantify, and address welfare concerns in zebrafish research, and to establish best practice guidelines.
摘要：
自从它在20世纪80年代作为模式生物被引入以来，斑马鱼（Daniorerio）在研究中的使用已在全球范围内扩展。尽管它现在广泛用于研究，保护斑马鱼伦理治疗的指导方针，特别是关于安乐死和人道的端点做法，保持不足。一个公认的例子是使用过量的甲磺酸三卡因（MS-222）作为安乐死斑马鱼的手段，无论人生阶段如何。在这项研究中,通过全国性的专家启发，我们详细介绍了大韩民国境内的斑马鱼研究实践，以及实施适当的安乐死方法作为人道终点的挑战，许多人选择低温休克。我们报告了当地专家共识，以建立改善斑马鱼福利和良好研究实践的国家指南。为国家准则提出了建议。一起来看,我们的发现广泛地提高了斑马鱼研究从业者的认识，在大韩民国的研究中提供斑马鱼的福利和治疗的准确说明，并倡导制定和执行国家指导方针。因此，我们的研究作为采用专家启发方法进行调查的模型是有用的，量化、并解决斑马鱼研究中的福利问题，并建立最佳实践准则。
