Abstract：
Corn silk is the stigma and style of corn and is rich in polysaccharides. Despite the extensive research on its polysaccharides, the hemostatic characteristics of effective parts and the related activities remain insufficiently explored. Corn silk polysaccharide (CSP) was extracted with hot water and purified using a diethylaminoethyl cellulose membrane. Then, it was separated with sephadex G-150 to obtain five fractions. These fractions were investigated for their potential in hemostasis, antioxidant, immune response, and anti-lung cancer activities. CSP-2, CSP-3, and CSP-4 significantly affected the coagulation indicators activated partial thromboplastin time (APTT) and thrombin time (TT) at 125-500 μg/mL. Corn silk flavonoids and saponins at 32.25 μg/mL significantly prolonged APTT, TT, and prothrombin time (PT). CSP-2, with potent antioxidant ability, approaches Vitamin C. At 25 μg/mL, CSPs nearly reached the phagocytosis of neutral red of lipopolysaccharides. The five fractions promoted the proliferation of RAW264.7 cells at 25-800 μg/mL and stimulated NO secretion at 25-100 μg/mL. CSP-2 also showed an 86 % inhibition rate effect on A549 at 200 μg/mL. These results indicate that CSP not only has hemostatic effects but also has immune and anti-lung cancer activities. Thus, it is a potential candidate compound with immune activity for managing bleeding in cancer.
摘要：
玉米丝是玉米的柱头和风格，富含多糖。尽管对其多糖进行了广泛的研究，有效部位的止血特性和相关活性仍未得到充分探索。用热水提取玉米丝多糖(CSP)并使用二乙基氨基乙基纤维素膜纯化。然后，将其用SephadexG-150分离以获得五个级分。研究了这些部分的止血潜力，抗氧化剂，免疫反应，和抗肺癌活性。CSP-2，CSP-3和CSP-4在125-500μg/mL时显著影响凝血指标活化部分凝血活酶时间（APTT）和凝血酶时间（TT）。玉米蚕丝黄酮和皂苷在32.25μg/mL时显著延长APTT,TT,和凝血酶原时间（PT）。CSP-2，具有强大的抗氧化能力，接近维生素C。在25μg/mL时，CSP几乎达到了脂多糖中性红的吞噬能力。5个组分在25-800μg/mL时促进RAW264.7细胞的增殖，在25-100μg/mL时刺激NO分泌。CSP-2在200μg/mL时对A549也显示出86%的抑制率效果。这些结果表明CSP不仅具有止血作用，而且具有免疫和抗肺癌活性。因此，它是一种具有免疫活性的候选化合物，可用于治疗癌症出血。
