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Abstract：
Staphylococcus aureus is a common clinical pathogen that causes various human infections. The aim of this study was to investigate the antibiotic susceptibility pattern, molecular epidemiological characteristics, and biofilm formation ability of S. aureus isolates from clinical specimens in Xiangyang and to analyze the correlation among them. A total of 111 non-duplicate S. aureus isolates were collected from the Affiliated Hospital of Hubei University of Arts and Science. All isolates were tested for antibacterial susceptibility. Methicillin-resistant S. aureus (MRSA) was identified by the mecA gene PCR amplification. All isolates were analyzed to determine their biofilm-forming ability using the microplate method. The biofilm-related gene was determined using PCR. SCCmec, MLST, and spa types of MRSA strains were performed to ascertain the molecular characteristics. Among the 111 S. aureus isolates, 45 (40.5%) and 66 (59.5%) were MRSA and MSSA, respectively. The resistance of MRSA strains to the tested antibiotics was significantly stronger than that of MSSA strains. All isolates were able to produce biofilm with levels ranging from strong (28.9%, 18.2%), moderate (62.2%, 62.1%), to weak (8.9%, 19.7%). Strong biofilm formation was observed in MRSA strains than in MSSA strains, based on percentages. There were dynamic changes in molecular epidemic characteristics of MRSA isolates in Xiangyang. SCCmecIVa-ST22-t309, SCCmecIVa-ST59-t437, and SCCmecIVa-ST5-t2460 were currently the main epidemic clones in this region. SCCmecIVa-ST5-t2460 and SCCmecIVa/III-ST22-t309 have stronger antibiotic resistance than SCCmecIVa-ST59-t437 strains, with resistance to 6 ~ 8 detected non-β-lactam antibiotics. The molecular epidemic and resistance attributes of S. aureus should be timely monitored, and effective measures should be adopted to control the clinical infection and spread of the bacteria.
摘要：
金黄色葡萄球菌是引起各种人类感染的常见临床病原体。这项研究的目的是调查抗生素的敏感性模式，分子流行病学特征，襄阳地区金黄色葡萄球菌临床分离株的生物膜形成能力,并对其相关性进行分析。从湖北文理学院附属医院收集到111株非重复金黄色葡萄球菌。测试所有分离株的抗菌敏感性。通过mecA基因PCR扩增鉴定耐甲氧西林金黄色葡萄球菌(MRSA)。使用微板方法分析所有分离物以确定其生物膜形成能力。使用PCR确定生物膜相关基因。SCCmec,MLST,并对MRSA菌株的spa类型进行了分析，以确定其分子特征。在111株金黄色葡萄球菌中，MRSA和MSSA分别为45例(40.5%)和66例(59.5%)，分别。MRSA菌株对试验抗生素的耐药性明显强于MSSA菌株。所有分离株都能够产生水平范围从strong（28.9%，18.2%），中等(62.2%，62.1%），至疲软（8.9%，19.7%）。在MRSA菌株中观察到比在MSSA菌株中更强的生物膜形成,基于百分比。襄阳地区MRSA分离株的分子流行特征存在动态变化。SCCmecIVa-ST22-t309，SCCmecIVa-ST59-t437和SCCmecIVa-ST5-t2460是目前该地区的主要流行克隆。SCCmecIVa-ST5-t2460和SCCmecIVa/III-ST22-t309比SCCmecIVa-ST59-t437菌株具有更强的抗生素抗性,检出6~8种非β-内酰胺类抗生素具有耐药性。应及时监测金黄色葡萄球菌的分子流行和耐药特性,并采取有效措施控制细菌的临床感染和传播。
