PDF(Pubmed)
Abstract：
Endogenous endophthalmitis caused by Gram-negative bacteria is an intra-ocular infection that can rapidly progress to irreversible loss of vision. While most endophthalmitis isolates are susceptible to antibiotic therapy, the emergence of resistant bacteria necessitates alternative approaches to combat intraocular bacterial proliferation. In this study the ability of predatory bacteria to limit intraocular growth of Pseudomonas aeruginosa, Serratia marcescens, and Staphylococcus aureus was evaluated in a New Zealand white rabbit endophthalmitis prevention model. Predatory bacteria Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus were able to reduce proliferation of keratitis isolates of P. aeruginosa and to a lesser extent S. marcescens. However, it was not able to significantly reduce the number of intraocular S. aureus, which is not a productive prey for these predatory bacteria, suggesting that the inhibitory effect on P. aeruginosa and S. marcescens requires active predation rather than an antimicrobial immune response. Similarly, UV-inactivated B. bacteriovorus were unable to prevent proliferation of P. aeruginosa. Together, these data indicate in vivo inhibition of Gram-negative bacteria proliferation within the intra-ocular environment by predatory bacteria.
摘要：
由革兰氏阴性菌引起的内源性眼内炎是一种眼内感染，可以迅速发展为不可逆的视力丧失。虽然大多数眼内炎分离株对抗生素治疗敏感，耐药细菌的出现需要其他方法来对抗眼内细菌的增殖。在这项研究中，掠夺性细菌限制铜绿假单胞菌眼内生长的能力，粘质沙雷菌,在新西兰白兔眼内炎预防模型中评估金黄色葡萄球菌。捕食性细菌Bdellovibriobacteriovorus和绿脓杆菌能够减少铜绿假单胞菌和较小程度的粘质链球菌的角膜炎分离株的增殖。然而,它不能显著减少眼内金黄色葡萄球菌的数量，它不是这些掠夺性细菌的生产性猎物，这表明对铜绿假单胞菌和粘质链球菌的抑制作用需要积极的捕食而不是抗菌免疫反应。同样，UV灭活的细菌芽孢杆菌不能防止铜绿假单胞菌的增殖。一起,这些数据表明捕食性细菌在眼内环境中体内抑制革兰氏阴性菌的增殖。
