PDF(Pubmed)
Abstract：
Screening a transposon-mutagenized soybean population led to the discovery of a recessively inherited chlorotic phenotype. This \"vir1\" phenotype results in smaller stature, weaker stems, and a smaller root system with smaller nodules. Genome sequencing identified 15 candidate genes with mutations likely to result in a loss of function. Amplicon sequencing of a segregating population was then used to narrow the list to a single candidate mutation, a single-base change in Glyma.07G102300 that disrupts splicing of the second intron. Single cell transcriptomic profiling indicates that this gene is expressed primarily in mesophyll cells and RNA sequencing data indicates it is upregulated in germinating seedlings by cold stress. Previous studies have shown that mutations to Os05g34040, the rice homolog of Glyma.07G102300, produced a chlorotic phenotype that was more pronounced in cool temperatures. Growing soybean vir1 mutants at lower temperatures also resulted in a more severe phenotype. In addition, transgenic expression of wild type Glyma.07G102300 in the knockout mutant of the Arabidopsis homolog At4930720 rescues the chlorotic phenotype, further supporting the hypothesis that the mutation in Glyma.07G102300 is causal of the vir1 phenotype.
摘要：
筛选转座子诱变的大豆种群导致发现了隐性遗传的褪绿表型。这种“vir1”表型导致身材矮小，较弱的茎，和较小的根系，结节较小。基因组测序鉴定了15个具有可能导致功能丧失的突变的候选基因。然后使用分离群体的扩增子测序将列表缩小为单个候选突变，Glyma.07G102300中的单碱基变化，破坏了第二个内含子的剪接。单细胞转录组谱分析表明该基因主要在叶肉细胞中表达，RNA测序数据表明它在发芽幼苗中通过冷胁迫上调。先前的研究表明，Glyma.07G102300的水稻同源物Os05g34040的突变产生了在凉爽温度下更为明显的褪绿表型。在较低温度下生长大豆vir1突变体也导致更严重的表型。此外,野生型Glyma.07G102300在拟南芥同源物At4930720的敲除突变体中的转基因表达挽救了褪绿表型，进一步支持Glyma.07G102300中的突变是vir1表型的原因的假设。
