PDF(Pubmed)
Abstract：
UNASSIGNED: To investigate the inhibition of Streptococcus mutans (S.mutans) and its biofilm by AgBr-nanoparticles (NP) @CTMAB (cetyltrimethyl-ammonium bromide) and evaluate the changes in Polymethyl methacrylate (PMMA)\'s surface roughness (Ra), microhardness, and flexural strength during prolonged immersion in AgBr-NP@CTMAB for application in the denture cleaning industry.
UNASSIGNED: The antibacterial activity of AgBr-NP@CTMAB against S.mutans was measured colony formation assay, OD600 and laser confocal microscopy. Changes in the specimens\' values for surface roughness, microhardness, and flexural strength (MPa) were measured after immersion solutions for 180 or 360 days.
UNASSIGNED: The AgBr-NP@CTMAB solution exhibited a robust antibacterial effect on planktonic S. mutans, with a minimum bactericidal concentration of 5 µg/mL. The 10 µg/mL AgBr-NP@CTMAB solution efficiently inhibited S. mutans biofilm formation. (2) No significant difference in surface roughness after immersion in AgBr-NP@CTMAB (10 µg/mL and 20 µg/mL) comparing with distilled water (P > 0.05) and Polident had significantly higher than distilled water (P < 0.05). There was a significant decrease in the surface hardness of the PMMA specimens that were immersed in the Polident compared with those in distilled water (P < 0.05). While, no significant differences in surface hardness after immersion in the AgBr-NP@CTMAB (P > 0.05). The result of flexural strength suggested that there was no statistically significant difference (P < 0.05) between AgBr-NP@CTMAB as well as Polident and water.
UNASSIGNED: AgBrNP@CTMAB can efficiently inhibit the growth of plankton S.mutans and biofilm formation, without affecting the flexural strength, microhardness, or surface roughness of PMMA. Therefore, AgBrNP@CTMAB holds promise as a new denture cleaning agent.
UNASSIGNED: The antibacterial activity of AgBr-NP@CTMAB against S.mutans was measured colony formation assay, OD600 and laser confocal microscopy. Changes in the specimens\' values for surface roughness, microhardness, and flexural strength (MPa) were measured after immersion solutions for 180 or 360 days.
UNASSIGNED: The AgBr-NP@CTMAB solution exhibited a robust antibacterial effect on planktonic S. mutans, with a minimum bactericidal concentration of 5 µg/mL. The 10 µg/mL AgBr-NP@CTMAB solution efficiently inhibited S. mutans biofilm formation. (2) No significant difference in surface roughness after immersion in AgBr-NP@CTMAB (10 µg/mL and 20 µg/mL) comparing with distilled water (P > 0.05) and Polident had significantly higher than distilled water (P < 0.05). There was a significant decrease in the surface hardness of the PMMA specimens that were immersed in the Polident compared with those in distilled water (P < 0.05). While, no significant differences in surface hardness after immersion in the AgBr-NP@CTMAB (P > 0.05). The result of flexural strength suggested that there was no statistically significant difference (P < 0.05) between AgBr-NP@CTMAB as well as Polident and water.
UNASSIGNED: AgBrNP@CTMAB can efficiently inhibit the growth of plankton S.mutans and biofilm formation, without affecting the flexural strength, microhardness, or surface roughness of PMMA. Therefore, AgBrNP@CTMAB holds promise as a new denture cleaning agent.
摘要：
■研究变形链球菌的抑制作用(S.mutans）及其生物膜通过AgBr-纳米颗粒（NP）@CTMAB（十六烷基三甲基溴化铵），并评估聚甲基丙烯酸甲酯（PMMA）的表面粗糙度（Ra）的变化，显微硬度,和长期浸入AgBr-NP@CTMAB中的弯曲强度，可用于义齿清洁行业。
■AgBr-NP@CTMAB对变形链球菌的抗菌活性通过集落形成试验测定，OD600和激光共聚焦显微镜。试样表面粗糙度值的变化,显微硬度,和弯曲强度(MPa)在浸渍溶液180或360天后测量。
■AgBr-NP@CTMAB溶液对浮游变形链球菌表现出强大的抗菌作用，最低杀菌浓度为5µg/mL。10µg/mLAgBr-NP@CTMAB溶液可有效抑制变形链球菌生物膜的形成。（2）AgBr-NP@CTMAB（10µg/mL和20µg/mL）浸泡后的表面粗糙度与蒸馏水相比没有显着差异（P>0.05），Polident的表面粗糙度明显高于蒸馏水（P<0.05）。与蒸馏水相比，浸入Polident中的PMMA样品的表面硬度显着降低（P<0.05）。同时,AgBr-NP@CTMAB浸泡后表面硬度无显著差异(P>0.05)。抗弯强度的结果表明，AgBr-NP@CTMAB以及Polident和水之间的差异无统计学意义（P<0.05）。
■AgBrNP@CTMAB可以有效抑制浮游生物S.mutans的生长和生物膜的形成，不影响弯曲强度，显微硬度,或PMMA的表面粗糙度。因此,AgBrNP@CTMAB有望成为新型义齿清洁剂。
■AgBr-NP@CTMAB对变形链球菌的抗菌活性通过集落形成试验测定，OD600和激光共聚焦显微镜。试样表面粗糙度值的变化,显微硬度,和弯曲强度(MPa)在浸渍溶液180或360天后测量。
■AgBr-NP@CTMAB溶液对浮游变形链球菌表现出强大的抗菌作用，最低杀菌浓度为5µg/mL。10µg/mLAgBr-NP@CTMAB溶液可有效抑制变形链球菌生物膜的形成。（2）AgBr-NP@CTMAB（10µg/mL和20µg/mL）浸泡后的表面粗糙度与蒸馏水相比没有显着差异（P>0.05），Polident的表面粗糙度明显高于蒸馏水（P<0.05）。与蒸馏水相比，浸入Polident中的PMMA样品的表面硬度显着降低（P<0.05）。同时,AgBr-NP@CTMAB浸泡后表面硬度无显著差异(P>0.05)。抗弯强度的结果表明，AgBr-NP@CTMAB以及Polident和水之间的差异无统计学意义（P<0.05）。
■AgBrNP@CTMAB可以有效抑制浮游生物S.mutans的生长和生物膜的形成，不影响弯曲强度，显微硬度,或PMMA的表面粗糙度。因此,AgBrNP@CTMAB有望成为新型义齿清洁剂。
