RESULTS: Here, we describe a method, based on sperm ion physiology that maintains L. pictus and Strongylocentrotus purpuratus sperm fertilizable for at least 5-10 weeks when stored at 0°C. We also describe a new method to cryopreserve sperm of both species. Sperm of both species can be frozen and thawed at least twice and still give rise to larvae that undergo metamorphosis.
CONCLUSIONS: The simple methods we describe work well for both species, achieving >90% embryo development and producing larvae that undergo metamorphosis to juvenile adults. We hope that these methods will be useful to others working on marine invertebrate sperm.
结果:这里,我们描述了一种方法,基于精子离子生理学,当储存在0°C时,可保持皮氏L.pictus和Strongylocentrotuspuricatus精子受精至少5-10周。我们还描述了一种冷冻保存这两个物种精子的新方法。两种物种的精子都可以冷冻和解冻至少两次,并且仍然会产生经历变态的幼虫。
结论:我们描述的简单方法对两个物种都有效,实现>90%的胚胎发育和产生的幼虫经历变态成虫。我们希望这些方法对其他研究海洋无脊椎动物精子的人有用。