PDF(Pubmed)
Abstract：
A novel conductive composite based on PEDOT:PSS, BSA, and Nafion for effective immobilization of acetic acid bacteria on graphite electrodes as part of biosensors and microbial fuel cells has been proposed. It is shown that individual components in the composite do not have a significant negative effect on the catalytic activity of microorganisms during prolonged contact. The values of heterogeneous electron transport constants in the presence of two types of water-soluble mediators were calculated. The use of the composite as part of a microbial biosensor resulted in an electrode operating for more than 140 days. Additional modification of carbon electrodes with nanomaterial allowed to increase the sensitivity to glucose from 1.48 to 2.81 μA × mM-1 × cm-2 without affecting the affinity of bacterial enzyme complexes to the substrate. Cells in the presented composite, as part of a microbial fuel cell based on electrodes from thermally expanded graphite, retained the ability to generate electricity for more than 120 days using glucose solution as well as vegetable extract solutions as carbon sources. The obtained data expand the understanding of the composition of possible matrices for the immobilization of Gluconobacter bacteria and may be useful in the development of biosensors and biofuel cells.
摘要：
一种基于PEDOT:PSS的新型导电复合材料,BSA,和Nafion作为生物传感器和微生物燃料电池的一部分，用于将乙酸细菌有效固定在石墨电极上。表明，在长时间接触过程中，复合材料中的各个组分对微生物的催化活性没有明显的负面影响。计算了两种水溶性介体存在下的异质电子传输常数的值。使用复合材料作为微生物生物传感器的一部分导致电极运行超过140天。用纳米材料对碳电极进行其他修饰可以将对葡萄糖的敏感性从1.48提高到2.81μA×mM-1×cm-2，而不会影响细菌酶复合物对底物的亲和力。呈现的复合物中的细胞，作为基于热膨胀石墨电极的微生物燃料电池的一部分，使用葡萄糖溶液和蔬菜提取物溶液作为碳源，保持了超过120天的发电能力。获得的数据扩展了对固定葡糖杆菌细菌的可能基质组成的理解，并且可能对开发生物传感器和生物燃料电池有用。
