Abstract：
Tick-borne encephalitis virus (TBEV) is a common virus in Europe and Asia, causing around 10,000 to 10,500 infections annually. It affects the central nervous system and poses threats to public health. However, the exact molecular mechanisms of TBE pathogenesis are not yet fully understood due to the complex interactions between the virus and its host. In this study, a comprehensive analysis was conducted to characterize the serum metabolome and proteome of adult patients infected with TBEV, in comparison to a control group of healthy individuals. Liquid chromatography tandem mass spectrometry (LC-MS) was employed to monitor metabolic and proteomic alternations throughout the progression of the disease, significant physiological changes associated with different stages of the disease were identified. A total of 44 proteins and 115 metabolites exhibited significantly alternations in the sera of patients diagnosed with TBE. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses of these metabolites and proteins revealed differential enrichment of genes associated with the extracellular matrix, complement binding, hemostasis, lipid metabolism, and amino acid metabolism between TBE patients and healthy controls. We gained valuable understanding of the specific metabolites implicated in the host\'s responses to TBE, establishing a basis for further research on TBE disease. SIGNIFICANCE: The current investigation revealed a comprehensive and systematic differences on TBE using LC-MS platform from human serum samples of TBE patients and healthy individuals providing the immune response to the invasion of TBE.
摘要：
蜱传脑炎病毒(TBEV)是欧洲和亚洲的一种常见病毒,每年造成约10,000至10,500例感染。它影响中枢神经系统并对公众健康构成威胁。然而,由于病毒与其宿主之间复杂的相互作用,TBE发病机制的确切分子机制尚未完全了解.在这项研究中,进行了全面分析，以表征感染TBEV的成年患者的血清代谢组和蛋白质组，与健康个体的对照组相比。液相色谱串联质谱（LC-MS）用于监测整个疾病进展过程中的代谢和蛋白质组变化。确定了与疾病不同阶段相关的显着生理变化。在诊断为TBE的患者的血清中，共有44种蛋白质和115种代谢物表现出明显的变化。基因本体论和京都百科全书的基因和基因组富集分析这些代谢物和蛋白质揭示了与细胞外基质相关的基因的差异富集。补体结合，止血,脂质代谢，以及TBE患者和健康对照者之间的氨基酸代谢。我们获得了与宿主对TBE反应有关的特定代谢物的宝贵理解，为进一步研究TBE病奠定基础。意义：目前的调查显示，使用LC-MS平台，TBE患者和健康个体的人血清样品对TBE的入侵提供免疫应答的TBE存在全面和系统的差异。
