Abstract：
BACKGROUND: S. pyogenes, is a primary pathogen that leads to pharyngitis and can also trigger severe conditions like necrotizing fasciitis and streptococcal toxic shock syndrome (STSS), often resulting in high mortality rates. Therefore, prompt identification and appropriate treatment of S. pyogenes infections are crucial in preventing the worsening of symptoms and alleviating the disease\'s impact.
RESULTS: In this study, a newly developed technique called multiple cross displacement amplification (MCDA) was employed to detect S. pyogenes,specifically targeting the speB gene, at a temperature of 63°C within 30 min. Then, an easily portable and user-friendly nanoparticles-based lateral flow biosensor (LFB) assay was introduced for the rapid analysis of MCDA products in just 2 min. The results indicated that the LFB offers greater objectivity compared to Malachite Green and is simpler than electrophoresis. The MCDA-LFB assay boasts a low detection limit of 200 fg and exhibits no cross-reaction with non-S. pyogenes strains. Among 230 clinical swab throat samples, the MCDA-LFB method identified 27 specimens as positive, demonstrating higher sensitivity compared to 23 samples detected positive by qPCR assay and 18 samples by culture. The only equipment needed for this assay is a portable dry block heater. Moreover, each MCDA-LFB test is cost-effective, priced at approximately $US 5.5.
CONCLUSIONS: The MCDA-LFB assay emerges as a straightforward, specific, sensitive, portable, and user-friendly method for the rapid diagnosis of S. pyogenes in clinical samples.
RESULTS: In this study, a newly developed technique called multiple cross displacement amplification (MCDA) was employed to detect S. pyogenes,specifically targeting the speB gene, at a temperature of 63°C within 30 min. Then, an easily portable and user-friendly nanoparticles-based lateral flow biosensor (LFB) assay was introduced for the rapid analysis of MCDA products in just 2 min. The results indicated that the LFB offers greater objectivity compared to Malachite Green and is simpler than electrophoresis. The MCDA-LFB assay boasts a low detection limit of 200 fg and exhibits no cross-reaction with non-S. pyogenes strains. Among 230 clinical swab throat samples, the MCDA-LFB method identified 27 specimens as positive, demonstrating higher sensitivity compared to 23 samples detected positive by qPCR assay and 18 samples by culture. The only equipment needed for this assay is a portable dry block heater. Moreover, each MCDA-LFB test is cost-effective, priced at approximately $US 5.5.
CONCLUSIONS: The MCDA-LFB assay emerges as a straightforward, specific, sensitive, portable, and user-friendly method for the rapid diagnosis of S. pyogenes in clinical samples.
摘要：
背景：S.化脓性,是导致咽炎的主要病原体，也可能引发坏死性筋膜炎和链球菌中毒性休克综合征（STSS）等严重疾病，往往导致高死亡率。因此,及时识别和适当治疗化脓性链球菌感染对于防止症状恶化和减轻疾病的影响至关重要。
结果：在这项研究中，一种新开发的称为多重交叉置换扩增（MCDA）的技术被用来检测化脓性链球菌，专门针对speB基因，在63°C的温度下在30分钟内。然后，引入了一种易于携带且用户友好的基于纳米颗粒的横向流生物传感器（LFB）测定法，可在短短2分钟内快速分析MCDA产品。结果表明，与孔雀石绿相比，LFB具有更大的客观性，并且比电泳更简单。MCDA-LFB测定具有200fg的低检测限，并且与非S没有交叉反应。化脓性菌株。在230个临床拭子咽喉样本中，MCDA-LFB方法确定27个样本为阳性，与qPCR检测阳性的23个样品和培养的18个样品相比，显示出更高的灵敏度。该测定所需的唯一设备是便携式干块加热器。此外，每个MCDA-LFB测试都具有成本效益，售价约5.5美元。
结论：MCDA-LFB测定是一种简单的，具体,敏感,便携式，和用户友好的方法，用于临床样品中化脓性链球菌的快速诊断。
结果：在这项研究中，一种新开发的称为多重交叉置换扩增（MCDA）的技术被用来检测化脓性链球菌，专门针对speB基因，在63°C的温度下在30分钟内。然后，引入了一种易于携带且用户友好的基于纳米颗粒的横向流生物传感器（LFB）测定法，可在短短2分钟内快速分析MCDA产品。结果表明，与孔雀石绿相比，LFB具有更大的客观性，并且比电泳更简单。MCDA-LFB测定具有200fg的低检测限，并且与非S没有交叉反应。化脓性菌株。在230个临床拭子咽喉样本中，MCDA-LFB方法确定27个样本为阳性，与qPCR检测阳性的23个样品和培养的18个样品相比，显示出更高的灵敏度。该测定所需的唯一设备是便携式干块加热器。此外，每个MCDA-LFB测试都具有成本效益，售价约5.5美元。
结论：MCDA-LFB测定是一种简单的，具体,敏感,便携式，和用户友好的方法，用于临床样品中化脓性链球菌的快速诊断。
