Abstract：
Cyantraniliprole is a novel insecticide recently introduced for rice pest control that may cause potential threats to the red swamp crayfish (Procambarus clarkii) in rice-crayfish coculture systems. In this study, we investigated the acute toxicity of cyantraniliprole against P. clarkii with a LC50 value of 149.77 mg/L (96 h), first. Some abnormal behaviors of P. clarkii treated with 125 mg/L cyantraniliprole, including incunabular hyperexcitability, imbalance, inactivity, and increased excretion were observed. Moreover, it was observed that exposure to 5 mg/L cyantraniliprole for 14 days resulted in histopathological alterations in abdominal muscle, gills, hepatopancreas, and intestines. Furthermore, exposure to 0.05 and 5 mg/L cyantraniliprole induced increased activities of several oxidative stress-related enzymes, which was verified by the upregulation of related genes. Additionally, dysregulation of the intestinal microbiota was determined via 16S rRNA sequencing. These results will provide the basis for the utilization of cyantraniliprole in the fields of rice-crayfish integrated system.
摘要：
Cyantraniliprole是最近引入的一种新型杀虫剂，用于水稻病虫害防治，可能会对水稻-小龙虾共培养系统中的红色沼泽小龙虾（Procambarusclarkii）造成潜在威胁。在这项研究中,我们研究了氰脲对克拉氏疟原虫的急性毒性，LC50值为149.77mg/L(96小时)，First.用125mg/L氰化蛋白治疗的克氏疟原虫的一些异常行为，包括incunabular过度兴奋，不平衡,不活动,并观察到排泄增加。此外，据观察，暴露于5毫克/升的蓝藻多糖14天导致腹部肌肉的组织病理学改变，ill,肝胰腺,和肠子。此外，暴露于0.05mg/L和5mg/L的氰脲可诱导几种氧化应激相关酶的活性增加，相关基因的上调证实了这一点。此外,通过16SrRNA测序确定肠道微生物群的失调。这些结果将为蓝藻在稻小龙虾一体化系统中的利用提供依据。
