Abstract：
BACKGROUND: Sturgeon is a popular aquaculture species in many countries. Its swim bladder is rich in collagen but has not yet been exploited scientifically.
RESULTS: Collagen peptides (CPs) prepared from sturgeon swim bladder by trypsinolysis had an average molecular weight of 528.5 Da and consisted of 407 peptides, 16.1% of the content of which was GFPGADGSAGPK. The CPs at 25 mg mL-1 extended the lifespan of Caenorhabditis elegans by 22.6%, which was significantly higher than the extension achieved by other hydrolysis methods and source materials. They also improved fitness-related traits (body size, motor capacity, oxidative stress, cell apoptosis, and epidermal barrier function), indicating prolonged healthspan. Transcriptome analysis showed that the effect was mediated by the mitogen-activated protein kinase pathway, which enhanced stress resistance, the insulin/IGF-1 pathway, which inhibited protein aggregation, and the NHR-80/FAT-6 pathway, which regulated lipid metabolism.
CONCLUSIONS: Collagen peptides from sturgeon swim bladder by trypsinolysis prolonged the lifespan and healthspan in C. elegans, and might be promising anti-aging agents. © 2024 Society of Chemical Industry.
RESULTS: Collagen peptides (CPs) prepared from sturgeon swim bladder by trypsinolysis had an average molecular weight of 528.5 Da and consisted of 407 peptides, 16.1% of the content of which was GFPGADGSAGPK. The CPs at 25 mg mL-1 extended the lifespan of Caenorhabditis elegans by 22.6%, which was significantly higher than the extension achieved by other hydrolysis methods and source materials. They also improved fitness-related traits (body size, motor capacity, oxidative stress, cell apoptosis, and epidermal barrier function), indicating prolonged healthspan. Transcriptome analysis showed that the effect was mediated by the mitogen-activated protein kinase pathway, which enhanced stress resistance, the insulin/IGF-1 pathway, which inhibited protein aggregation, and the NHR-80/FAT-6 pathway, which regulated lipid metabolism.
CONCLUSIONS: Collagen peptides from sturgeon swim bladder by trypsinolysis prolonged the lifespan and healthspan in C. elegans, and might be promising anti-aging agents. © 2024 Society of Chemical Industry.
摘要：
背景：St鱼在许多国家是一种流行的水产养殖物种。它的游泳膀胱富含胶原蛋白，但尚未被科学开发。
结果：通过胰蛋白酶分解来自st鱼游泳膀胱的胶原蛋白肽（CPs）的数均分子量为528.5Da，由407个肽组成，其中GFPGADGSAGPK含量为16.1%。25mg/mL的CPs将秀丽隐杆线虫的寿命延长了22.6%,显著高于其他水解方法和原料。他们还改善了与健身相关的特征(体型，电机容量,氧化应激，细胞凋亡和表皮屏障功能），表明健康时间延长。Further,转录组分析表明MAPK通路介导的增强抗逆性,胰岛素/IGF-1途径抑制蛋白质聚集，和NHR-80/FAT-6通路调节脂质代谢。
结论：通过胰蛋白酶分解从st鱼游泳膀胱中提取的CPs延长了秀丽隐杆线虫的寿命和健康状况，可能是很有前途的抗衰老剂。本文受版权保护。保留所有权利。
结果：通过胰蛋白酶分解来自st鱼游泳膀胱的胶原蛋白肽（CPs）的数均分子量为528.5Da，由407个肽组成，其中GFPGADGSAGPK含量为16.1%。25mg/mL的CPs将秀丽隐杆线虫的寿命延长了22.6%,显著高于其他水解方法和原料。他们还改善了与健身相关的特征(体型，电机容量,氧化应激，细胞凋亡和表皮屏障功能），表明健康时间延长。Further,转录组分析表明MAPK通路介导的增强抗逆性,胰岛素/IGF-1途径抑制蛋白质聚集，和NHR-80/FAT-6通路调节脂质代谢。
结论：通过胰蛋白酶分解从st鱼游泳膀胱中提取的CPs延长了秀丽隐杆线虫的寿命和健康状况，可能是很有前途的抗衰老剂。本文受版权保护。保留所有权利。
