Abstract：
Coastal ecosystems are vital for maintaining the biodiversity and human livelihoods, but they are increasingly subjected to anthropogenic pressures, including pollution from various sources. Present work intends to assess the possible threats in coastal ecosystem as well as coastal fish species, in particular, through haematological parameters caused due to exposure of environmental contaminants like polycyclic aromatic hydrocarbons (PAHs), potentially toxic metals (PTMs), etc. This study analysed the haematological parameters and probable toxicity levels in two important coastal fish species, viz., Mystus sp. and Mugil sp. widely available in Digha coastal belt. Different haematological parameters, such as WBCs (White Blood Cells), Lym (Lymphocytes), Gran (Granulocytes), Mid (Monocytes), RBCs (Red Blood Cells), HCT (Haematocrit) value, MCV (Mean Corpuscular Volume), MCH (Mean Corpuscular Haemoglobin), MCHC (Mean Corpuscular Haemoglobin Concentration), RDW- CV (Red Cells Distribution Width-Co-efficient of Variation), RDW- SD (Red Cells Distribution Width-Standard Deviation), PLT (Total Platelet Count), MPV (Mean Platelet Volume), PDW- SD (Platelet Distribution Width-Standard Deviation), PDW- CV (Platelet Distribution Width-Co-efficient of Variation), PCT (Plateletcrit), PLCR (Platelet Large Cell Ratio), PLCC (Platelet Large Cell Count) and many others were measured directly through Erba H360 Haematology Analyser, simultaneously air dried blood smear was stained by Haematoxylin-Eosin(H-E) and Giemsa stain for assessing morphometric alterations of RBCs, WBCs, platelets as well as to determine the differential counts of WBCs by observing through Leica DM2000 microscope. Evidence of several abnormalities in the erythrocyte\'s nucleus (ENAs) and the abundance of abnormal celled erythrocytes (ECAs), carcinoma (lymphoproliferative disorder, polycythaemia vera, Hodgkin lymphoma and non-Hodgkin lymphoma), elevation of WBCs content, Lym %(Lymphocyte percentage), Eo(Eosinophils), monocytes, HCT and gross depletion of Ne(Neutrophils), basophils, and PLCR levels indicated a sign of major impact of contamination to two intoxicated fishes which may also affect the human being through food chain and may result into leukaemia in mammalian species, finally. However, comprehensive evaluation of the long-term impacts of the contaminants like PAHs and/or PTMs, etc., on fish populations, human health risk and coastal ecosystem is required to be addressed.
摘要：
沿海生态系统对于维持生物多样性和人类生计至关重要，但是他们越来越受到人为的压力，包括各种来源的污染。当前的工作旨在评估沿海生态系统以及沿海鱼类物种的可能威胁，特别是,通过由于接触多环芳烃（PAHs）等环境污染物而引起的血液学参数，潜在有毒金属(PTM)，等。这项研究分析了两种重要的沿海鱼类的血液学参数和可能的毒性水平，viz.,Mystussp.和Mugilsp.在Digha沿海地带广泛可用。不同的血液学参数，如WBC（白细胞），Lym（淋巴细胞），Gran（粒细胞），Mid（单核细胞），红细胞（红细胞），HCT（血细胞比容），MCV（平均红细胞体积），MCH(平均红细胞血红蛋白),MCHC（平均红细胞血红蛋白浓度），RDWCV(红细胞分布宽度-变异系数)，RDWSD（红细胞分布宽度-标准偏差），血小板总数(PLT),MPV（平均血小板体积），PDWSD（血小板分布宽度-标准偏差），PDWCV(血小板分布宽度-变异系数),PCT(Plateletcrit),PLCR（血小板大细胞比率），PLCC（血小板大细胞计数）通过ErbaH360血液分析仪直接测量，同时用Haematoxiline-Eosin和Giemsa染色对空气干燥的血液涂片进行分类计数，并通过Leicadm2000显微镜观察。红细胞细胞核（ENA）和异常细胞红细胞（ECA）丰度的若干异常的证据，癌（淋巴增生性疾病，真性红细胞增多症,霍奇金淋巴瘤和非霍奇金淋巴瘤），WBC的海拔，Lym%,EO,单核细胞,HCT和Ne的总消耗，嗜碱性粒细胞,和PLCR水平表明污染对两种中毒鱼类的重大影响的迹象，这也可能通过食物链影响人类，并可能导致哺乳动物物种的白血病，finally.然而,对PAHs和/或PTM等污染物的长期影响进行综合评估，等。,关于鱼类种群，人类健康风险和沿海生态系统需要得到解决。
