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Abstract：
The genetic variation and population structure of gene N (nucleocapsid) and part of gene L (replicase) from 13 eggplant mottle dwarf virus (EMDV) isolates from Spain were evaluated and compared with sequences of EMDV isolates from other countries retrieved from GenBank. Phylogenetic inference of part of gene L showed three main clades, one containing an EMDV isolate from Australia and the other two containing isolates from Iran and Europe, as well as four subclades. EMDV isolates from Spain were genetically very similar and grouped in a subclade together with one isolate from Germany and one from the UK. No new recombination events were detected in addition to one recombination previously reported, suggesting that recombination is rare for EMDV. The comparison of synonymous and non-synonymous rates showed that negative selection played an important role, and only two codons were under positive selection. Genetic differentiation (Fst test), phylogenetic and nucleotide diversity analyses suggest a unique introduction of EMDV to Spain and low gene flow with other countries. In contrast, Greece and Italy showed diverse populations with high gene flow between both.
摘要：
对来自西班牙的13个茄子斑驳矮病毒（EMDV）分离株的基因N（核衣壳）和部分基因L（复制酶）的遗传变异和种群结构进行了评估，并与从GenBank检索的其他国家EMDV分离株的序列进行了比较。部分L基因的系统发育推断显示了三个主要分支，一个含有来自澳大利亚的EMDV分离株，另两个含有来自伊朗和欧洲的分离株，以及四个分支。来自西班牙的EMDV分离株在遗传上非常相似，并与来自德国的一种分离株和来自英国的一种分离株一起分组在一个子分支中。除了先前报道的一次重组外，没有检测到新的重组事件。这表明重组对于EMDV是罕见的。同义率和非同义率的比较表明，负选择发挥了重要作用，只有两个密码子处于正选择状态。遗传分化（Fst检验），系统发育和核苷酸多样性分析表明，EMDV独特地引入了西班牙，并且与其他国家的基因流量较低。相比之下,希腊和意大利显示出不同的种群，两者之间的基因流量很高。
