■thalictrumfargesii是一种药用植物,属于毛茸茸科的thalictrum属,已在中国和印度的喜马拉雅地区用于草药。由于该物种与该属中的其他物种的形态相似性,因此在分类学上具有挑战性。因此,这个物种的草药经常掺假,替换,或与其他物种混合,从而危及消费者安全。
■本研究旨在使用IlluminaHiSeq2500平台对T.fargesii的整个叶绿体(cp)基因组进行测序和组装,以更好地了解基因组结构,基因组成,和Thalictrum内的系统发育关系。
■cp基因组长155,929bp,包含大的单拷贝(85,395bp)和小的单拷贝(17,576bp)区域,由一对反向重复区(26,479bp)隔离,形成四部结构。cp基因组包含133个基因,包括88个蛋白质编码基因(PCGs),37个tRNA基因,和8个rRNA基因。此外,这个基因组包含64个编码20个氨基酸的密码子,其中最优选的是丙氨酸和亮氨酸。我们确定了68个SSR,27次长重复,和cp基因组中242个高置信度C到URNA编辑位点。此外,我们在T.fargesii的cp基因组中发现了七个不同的热点区域,其中ndhD-psaC和rpl16-rps3可用于开发分子标记,以鉴定民族药物种类及其污染物。与该属中其他八个物种的比较研究表明,pafI和rps19在T.fargesii的cp基因组中具有高度可变的位点。此外,两个特殊功能,(i)IRA-SSC边界处的ycf1基因的最短长度和(ii)IRA-LSC连接处的rps19片段与trnH之间的距离,将T.fargesii的cp基因组与该属内其他物种的基因组区分开来。此外,系统发育分析显示,法格氏T.fargesii与T.tenue和T.petaloidium密切相关。
■考虑到所有这些证据,我们的发现提供了至关重要的分子和进化信息,这些信息可能在进一步的物种鉴定中发挥重要作用,进化,以及对T.fargesii的系统发育研究。
UNASSIGNED: Thalictrum fargesii is a medicinal plant belonging to the genus Thalictrum of the Ranunculaceae family and has been used in herbal medicine in the Himalayan regions of China and India. This species is taxonomically challenging because of its morphological similarities to other species within the genus. Thus, herbal drugs from this species are frequently adulterated, substituted, or mixed with other species, thereby endangering consumer safety.
UNASSIGNED: The present study aimed to sequence and assemble the entire chloroplast (cp) genome of T. fargesii using the Illumina HiSeq 2500 platform to better understand the genomic architecture, gene composition, and phylogenetic relationships within the Thalictrum.
UNASSIGNED: The cp genome was 155,929 bp long and contained large single-copy (85,395 bp) and small single-copy (17,576 bp) regions that were segregated by a pair of inverted repeat regions (26,479 bp) to form a quadripartite structure. The cp genome contains 133 genes, including 88 protein-coding genes (PCGs), 37 tRNA genes, and 8 rRNA genes. Additionally, this genome contains 64 codons that encode 20 amino acids, the most preferred of which are alanine and leucine. We identified 68 SSRs, 27 long repeats, and 242 high-confidence C-to-U RNA-editing sites in the cp genome. Moreover, we discovered seven divergent hotspot regions in the cp genome of T. fargesii, among which ndhD-psaC and rpl16-rps3 may be useful for developing molecular markers for identifying ethnodrug species and their contaminants. A comparative study with eight other species in the genus revealed that pafI and rps19 had highly variable sites in the cp genome of T. fargesii. Additionally, two special features, (i) the shortest length of the ycf1 gene at the IRA-SSC boundary and (ii) the distance between the rps19 fragment and trnH at the IRA-LSC junction, distinguish the cp genome of T. fargesii from those of other species within the genus. Furthermore, phylogenetic analysis revealed that T. fargesii was closely related to T. tenue and T. petaloidium.
UNASSIGNED: Considering all these lines of evidence, our findings offer crucial molecular and evolutionary information that could play a significant role in further species identification, evolution, and phylogenetic studies on T. fargesii.