PDF(Pubmed)
Abstract：
As one of the most important environmental signals, photoperiod plays a crucial role in regulating the growth, metabolism, and survival of organisms. The photoperiod shifts with the transition of the seasons. The difference in photoperiod between summer and winter is the greatest under natural conditions. However, the effect of photoperiod on Huanghe carp (Cyprinus carpio haematopterus) was paid little attention. We investigated the impact of artificial manipulation of seasonal photoperiod on Huanghe carp by integrating growth performance, intestinal flora, and intestinal metabolome.
We conducted an 8-week culture experiment with summer photoperiod (14 h light:10 h dark, n = 60) as the control group and winter photoperiod (10 h light:14 h dark, n = 60) based on the natural laws.
Winter photoperiod provokes significant weight increases in Huanghe carp. The altered photoperiod contributed to a significant increase in triglyceride and low-density lipoprotein cholesterol levels and the gene expressions of lipid metabolism in the intestine of Huanghe carp. 16s rDNA sequencing revealed that winter photoperiod diminished intestinal flora diversity and altered the abundance. Specifically, the relative abundances of Fusobacteria and Acidobacteriota phyla were higher but Proteobacteria, Firmicutes, and Bacteroidetes phyla were reduced. Analogously, photoperiodic changes induced a significant reduction in the Pseudomonas, Vibrio, Ralstonia, Acinetobacter, and Pseudoalteromonas at the genus level. Additionally, metabolomics analysis showed more than 50% of differential metabolites were associated with phospholipids and inflammation. Microbiome and metabolome correlation analyses revealed that intestinal microbe mediated lipid metabolism alteration.
The winter photoperiod induced intestinal flora imbalance and lipid metabolism modification, ultimately affecting the growth of Huanghe carp. This study provides new insights into the effects of seasonal photoperiodic alteration on the well-being of fish.
We conducted an 8-week culture experiment with summer photoperiod (14 h light:10 h dark, n = 60) as the control group and winter photoperiod (10 h light:14 h dark, n = 60) based on the natural laws.
Winter photoperiod provokes significant weight increases in Huanghe carp. The altered photoperiod contributed to a significant increase in triglyceride and low-density lipoprotein cholesterol levels and the gene expressions of lipid metabolism in the intestine of Huanghe carp. 16s rDNA sequencing revealed that winter photoperiod diminished intestinal flora diversity and altered the abundance. Specifically, the relative abundances of Fusobacteria and Acidobacteriota phyla were higher but Proteobacteria, Firmicutes, and Bacteroidetes phyla were reduced. Analogously, photoperiodic changes induced a significant reduction in the Pseudomonas, Vibrio, Ralstonia, Acinetobacter, and Pseudoalteromonas at the genus level. Additionally, metabolomics analysis showed more than 50% of differential metabolites were associated with phospholipids and inflammation. Microbiome and metabolome correlation analyses revealed that intestinal microbe mediated lipid metabolism alteration.
The winter photoperiod induced intestinal flora imbalance and lipid metabolism modification, ultimately affecting the growth of Huanghe carp. This study provides new insights into the effects of seasonal photoperiodic alteration on the well-being of fish.
摘要：
■作为最重要的环境信号之一，光周期在调节生长中起着至关重要的作用，新陈代谢，和生物的生存。光周期随着季节的过渡而变化。在自然条件下，夏季和冬季之间的光周期差异最大。然而,光周期对黄河鲤鱼（Cyprinuscarpiohematopterus）的影响很少受到关注。我们通过整合生长性能，研究了人工操纵季节性光周期对黄河鲤鱼的影响，肠道菌群，和肠道代谢组。
■我们用夏季光周期进行了为期8周的培养实验（14小时光照：10小时黑暗，n=60）作为对照组和冬季光周期（10h光照：14h黑暗，n=60）基于自然规律。
■冬季光周期引起黄河鲤鱼体重的显着增加。光周期的改变导致黄河鲤鱼肠道甘油三酯和低密度脂蛋白胆固醇水平以及脂质代谢基因表达的显着增加。16srDNA测序表明，冬季光周期减少了肠道菌群多样性并改变了丰度。具体来说,梭菌和酸性杆菌的相对丰度较高，而变形杆菌，Firmicutes,和拟杆菌门减少。类似地,光周期变化诱导假单胞菌的显著减少，弧菌,Ralstonia,不动杆菌,和属水平的假交替单胞菌。此外,代谢组学分析显示,超过50%的差异代谢物与磷脂和炎症相关.微生物组和代谢组相关性分析显示肠道微生物介导脂质代谢改变。
■冬季光周期引起的肠道菌群失调和脂质代谢改变，最终影响黄河鲤鱼的生长。这项研究为季节性光周期变化对鱼类福祉的影响提供了新的见解。
■我们用夏季光周期进行了为期8周的培养实验（14小时光照：10小时黑暗，n=60）作为对照组和冬季光周期（10h光照：14h黑暗，n=60）基于自然规律。
■冬季光周期引起黄河鲤鱼体重的显着增加。光周期的改变导致黄河鲤鱼肠道甘油三酯和低密度脂蛋白胆固醇水平以及脂质代谢基因表达的显着增加。16srDNA测序表明，冬季光周期减少了肠道菌群多样性并改变了丰度。具体来说,梭菌和酸性杆菌的相对丰度较高，而变形杆菌，Firmicutes,和拟杆菌门减少。类似地,光周期变化诱导假单胞菌的显著减少，弧菌,Ralstonia,不动杆菌,和属水平的假交替单胞菌。此外,代谢组学分析显示,超过50%的差异代谢物与磷脂和炎症相关.微生物组和代谢组相关性分析显示肠道微生物介导脂质代谢改变。
■冬季光周期引起的肠道菌群失调和脂质代谢改变，最终影响黄河鲤鱼的生长。这项研究为季节性光周期变化对鱼类福祉的影响提供了新的见解。
