PDF(Pubmed)
Abstract：
We investigated the diversity and occurrence of wood wasps in Lithuania and determined communities of associated fungi. Trapping of wood wasps resulted in three different species, including Sirex juvencus, Urocerus gigas, and Tremex fuscicornis. Fungal culturing from adult females of T. fuscicornis mainly resulted in fungi from the genera Penicillium and Trichoderma. High-throughput sequencing of ITS2 rDNA resulted in 59,797 high-quality fungal sequences, representing 127 fungal OTUs. There were 93 fungal OTUs detected in U. gigas, 66 in S. juvencus, and 10 in T. fuscicornis. The most common fungi were Fusarium sporotrichioides (63.1% of all fungal sequences), Amylostereum chailletii (14.9%), Penicillium crustosum (7.8%), Microascus sp. 2261_4 (5.0%), and Pithoascus ater (2.1%). Among these, only A. chailletii was found in all three insect species with the highest relative abundance in U. gigas (15.2%), followed by S. juvencus (7.7%), and the lowest in T. fuscicornis (0.3%) (p < 0.0003). Correspondence analysis of fungal communities showed a distant placement of different species of wood wasps, indicating that fungal communities in each of these were largely different. In conclusion, the study showed that the economically important tree pathogen A. chailletii was among the most common fungal OTUs vectored by siricid wood wasps.
摘要：
我们调查了立陶宛木蜂的多样性和发生情况，并确定了相关真菌的群落。捕获木蜂导致三种不同的物种，包括SirexJuvencus,尿路gigas,和Tremexfuscicornis.从T.fuscicornis的成年雌性中培养真菌主要导致来自青霉和木霉属的真菌。ITS2rDNA的高通量测序产生了59,797个高质量的真菌序列，代表127个真菌OTU。在U.gigas中检测到93种真菌OTU，66在S.Juvencus，和10在T.fuscicornis。最常见的真菌是镰刀菌（占所有真菌序列的63.1％），赤霉病(14.9%)，地壳青霉(7.8%),微曲霉。2261_4（5.0%），和猪曲霉水（2.1％）。其中,在U.gigas中相对丰度最高的所有三种昆虫物种中仅发现了A.chailletii（15.2％），其次是S.juvencus(7.7%)，和最低的T.fuscicornis（0.3%）（p<0.0003）。真菌群落的对应分析表明，不同种类的木黄蜂在远处放置，表明这些真菌群落在很大程度上是不同的。总之,该研究表明，经济上重要的树木病原体A.chailletii是最常见的真菌OTUs，由silicid木黄蜂传播。
