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Abstract：
Neutralizing capacity measurement (NCM) of soluble ABH substances (SAS) in plasma was assessed to guide the selection of the appropriate ABO group of fresh-frozen plasma (FFP) for plasma exchange (PE) in blood group O recipients with ABO-incompatible transplantations. Neutralizing capacity was assessed by measuring anti-A and/or anti-B titers in samples comprising one unit of O FFP and 10 O EDTA plasma samples and subtracting the binary logarithm of the titer in each group with a saline dilution. Ten EDTA plasma samples with Lewis b (Leb) antigen positivity and 10 sets of pooled FFP from each blood group were used as diluents. In O FFP, the NCM values (mean±SD) were 3.4±0.52 (2.6±0.52) and 2.6±0.52 (1.5±0.3) in B and AB for IgM (total antibody) anti-B (both P<0.001), and in the 10 O EDTA plasma samples, they were 3.9±0.88 (3.1±0.88) and 3.2±0.79 (2.4±0.97) for IgM (P=0.0013) and total anti-B (P=0.025), respectively. In vitro analysis revealed that B FFP is more effective than AB FFP in reducing IgM and total anti-B antibody titers in O recipients, regardless of Leb antigen positivity.
摘要：
评估血浆中可溶性ABH物质（SAS）的中和能力（NCM），以指导选择合适的ABO组新鲜冷冻血浆（FFP）进行血浆置换（PE）。通过测量包含一个单位的OFFP和IOOEDTA血浆样品的样品中的抗A和/或抗B滴度并用盐水稀释减去每组中的滴度的二元对数来评估中和能力。将来自每个血型的10个具有Lewisb(Leb)抗原阳性的EDTA血浆样品和10组汇集的FFP用作稀释剂。在OFFP中，对于IgM（总抗体）抗B，B和AB的NCM值（平均值±SD）分别为3.4±0.52（2.6±0.52）和2.6±0.52（1.5±0.3）（均P<0.001），在10OEDTA血浆样本中，IgM（P=0.0013）和总抗B（P=0.025）分别为3.9±0.88（3.1±0.88）和3.2±0.79（2.4±0.97），分别。体外分析表明，BFFP比ABFFP更有效地降低O受体的IgM和总抗B抗体滴度，无论Leb抗原阳性。
