Abstract：
The potentially toxic effects of emerging pollutant mixtures often deviate from the individual compound effects, presenting additive, synergistic, or agonistic interactions. This study delves into the complex world of emerging pollutants\' mixtures, with a particular focus on their potential impact on unsaturated lipid DOPC (1,2-dioleoyl-sn-glycerol-3-phosphocholine) structured as both monolayers and bilayers, which are valuable tools for mimicking cell membranes. Specifically, we examine the effects of two common types of pollutants: antibiotics (amoxicillin) and dyes (methylene blue). Utilizing Langmuir monolayers, our research reveals a synergistic effect within the pollutant mixture, as evidenced by pressure-area isotherms and polarization-modulated infrared reflection absorption spectroscopy. We identify the specific chemical interactions contributing to this synergistic effect. Furthermore, through contrast phase microscopy experiments on giant unilamellar vesicles (bilayer system), we find that the individual pollutants and the mixture exhibit similar molecular effects on the bilayer, revealing that the molecular size is a key factor in the bilayer-mixture of pollutant interaction. This highlights the importance of considering molecular size in the interactions with bilayer systems. In summary, our research dissects the critical factors of chemical interactions and molecular size concerning the effects of pollutants on DOPC, serving as simplified models of cell membranes. This study underscores the significance of comprehending the molecular effects of emerging pollutants on human health and the development of models for exploring their intricate interactions with cell membranes.
摘要：
新出现的污染物混合物的潜在毒性效应往往偏离单独的化合物效应,呈递添加剂，协同，或激动的相互作用。这项研究深入研究了新兴污染物混合物的复杂世界，特别关注它们对不饱和脂质DOPC（1,2-二油酰基-sn-甘油-3-磷酸胆碱）的潜在影响，结构为单层和双层，这是模仿细胞膜的有价值的工具。具体来说,我们研究了两种常见类型的污染物的影响：抗生素（阿莫西林）和染料（亚甲蓝）。利用朗缪尔单层膜，我们的研究揭示了污染物混合物内的协同作用，压力面积等温线和偏振调制红外反射吸收光谱证明了这一点。我们确定了有助于这种协同效应的特定化学相互作用。此外，通过巨大的单层囊泡（双层系统）的对比相位显微镜实验，我们发现单个污染物和混合物在双层上表现出相似的分子效应，揭示了分子大小是污染物相互作用的双层混合物的关键因素。这突出了在与双层系统的相互作用中考虑分子大小的重要性。总之,我们的研究剖析了污染物对DOPC影响的化学相互作用和分子大小的关键因素，作为细胞膜的简化模型。这项研究强调了理解新兴污染物对人类健康的分子效应以及探索其与细胞膜复杂相互作用的模型的开发的重要性。
