PDF(Pubmed)
Abstract：
Faced with rising threats of terrorism, environmental and health risks, achieving sensitive and selective detection of peroxide-based explosives (PEs) has become a global focus. In this study, a turn-on fluorescent probe (BOD) based on benzil (H2O2-recognition element) and 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) derivative (fluorophore) was developed to sensitively and specifically detect hydrogen peroxide (H2O2). The synthesized BOD had a very weak fluorescence due to intramolecular donor-excited photo-induced electron transfer (d-PET) effect; however, it could emit a strong fluorescence since H2O2 selectively oxidized the benzil moiety and released free BODIPY fluorophore (BOD-COOH). As a result, the proposed BOD detected H2O2 in linear detection ranged from 25 to 125 µM with a detection limit of 4.41 µM. Meanwhile, the proposed BOD showed good selectivity toward H2O2, which is not affected by other common reactive oxygen species (ROS) and ions from explosive residues. In addition, a blue shift from 508 to 498 nm was observed in the absorption spectra upon addition of H2O2. More importantly, the BOD was successfully applied for rapid detection of H2O2 vapor with good sensitivity (down to 7 ppb), which holds great potential for practical use in public safety, forensic analysis and environmental monitoring.
摘要：
面对不断上升的恐怖主义威胁，环境和健康风险，实现对基于过氧化物的爆炸物(PEs)的灵敏和选择性检测已成为全球关注的焦点。在这项研究中,基于苯并（H2O2识别元素）和4,4-二氟-4-硼-3a的开启荧光探针（BOD），4a-二氮杂-s-茚并苯（BODIPY）衍生物（荧光团）被开发用于灵敏和特异性地检测过氧化氢（H2O2）。由于分子内供体激发的光致电子转移(d-PET)效应,合成的BOD具有非常弱的荧光;它可以发出强烈的荧光，因为H2O2选择性氧化苯偶酰部分并释放游离的BODIPY荧光团（BOD-COOH）。因此,拟议的BOD在线性检测中检测H2O2的范围为25至125µM，检测限为4.41µM。同时,拟议的BOD对H2O2具有良好的选择性，不受其他常见活性氧（ROS）和爆炸性残留物离子的影响。此外,添加H2O2后，在吸收光谱中观察到从508到498nm的蓝移。更重要的是,BOD成功地应用于快速检测H2O2蒸气，具有良好的灵敏度（低至7ppb），在公共安全中具有巨大的实际应用潜力，法医分析和环境监测。
