Abstract：
The aim of this study was to investigate the presence and genetic characteristics of Bartonella quintana in pet cats from Urmia City, located in the northwest of Iran. Blood samples were collected from 200 cats, and their age, gender, and breed were noted. Nested-PCR and sequencing were used to identify B. quintana in positive samples, and the ftsZ gene sequences were analyzed using BioEdit software. The gene sequence obtained in this study exhibited 100.00 % similarity to reference sequences in the GenBank® database, and a phylogenetic tree was constructed using MEGA11. The results revealed that 15 % of the cats (30 out of 200 blood samples) tested positive for the B. quintana gene, with a 95 % confidence interval of 10.71 % to 20.61 %.
摘要：
这项研究的目的是调查乌尔米亚市宠物猫中巴尔通菌的存在和遗传特征。位于伊朗西北部。收集了200只猫的血样，和他们的年龄,性别,并注意到品种。巢式PCR和测序用于鉴定阳性样品中的金塔纳芽孢杆菌。使用BioEdit软件分析ftsZ基因序列。本研究中获得的基因序列与GenBank®数据库中的参考序列具有100.00%的相似性，并使用MEGA11构建了系统发育树。结果显示，15%的猫(200份血样中的30份)检测出的金塔纳芽孢杆菌基因呈阳性，95%置信区间为10.71%至20.61%。
