Abstract：
Weight regain subsequent to weight reduction resulting from dietary interventions represents a prevalent phenomenon recognized as \"Yo-yo dieting.\" However, the impact of prolonged Yo-yo dieting on health, especially in relation to the aging process, remains poorly understood. This study aimed to investigate the influence of Yo-yo dieting on the aging process in male Drosophila melanogaster that have been exposed to a high-calorie (HC) diet. Fruit flies were fed with either a consistent HC diet or an alternating regimen of HC and low-calorie diets every 3 days (referred to as \"Yo-yo dieting\") for a total of 24 days. Biochemical assays were utilized to quantify levels of oxidative stress and activities of the mitochondrial respiratory chain complexes. The frozen section staining method was employed to assess the presence of lipid droplets, reactive oxygen species, cellular viability, and mitochondrial abundance in tissues. Additionally, we examined the expression of key regulators involved in mitochondrial dynamics and biogenic signaling pathways. Yo-yo dieting resulted in an extension of the fruit flies\' lifespan, concomitant with reduced body weight, decreased body protein content, and lower triglyceride levels compared to continuous a HC diet feeding. Furthermore, Yo-yo dieting ameliorated impairments in motility and intestinal barrier function. Importantly, it improved mitochondrial function and upregulated the expression of essential mitochondrial fusion proteins, namely mitofusin 1 and mitofusin 2, optic atrophy 1, and peroxisome proliferator-activated receptor-γ coactivator-1α. Therefore, the practice of Yo-yo dieting extends the lifespan of fruit flies by modulating mitochondrial dynamics and the associated biogenic signaling pathways.
摘要：
饮食干预导致体重减轻后的体重恢复代表了公认的“溜溜球节食”的普遍现象。\"然而,长期溜溜球节食对健康的影响，特别是在衰老过程中，仍然知之甚少。这项研究旨在研究Yo-yo节食对暴露于高热量（HC）饮食的雄性果蝇衰老过程的影响。果蝇每三天以一致的HC饮食或HC和低热量饮食的交替方案喂养（称为“溜溜球节食”），共24天。生化测定用于量化氧化应激水平和线粒体呼吸链复合物的活性。采用冷冻切片染色方法评估脂滴的存在，活性氧，细胞活力，和组织中的线粒体丰度。此外,我们检测了参与线粒体动力学和生物信号通路的关键调节因子的表达.溜溜球节食延长了果蝇的寿命，伴随着体重减轻，身体蛋白质含量降低，与连续的HC饮食喂养相比，甘油三酯水平较低。此外，悠悠节食改善了运动和肠屏障功能的损伤。重要的是,它改善了线粒体功能并上调了必需的线粒体融合蛋白的表达，即mitofusin1和mitofusin2，视神经萎缩1（Opa1），和过氧化物酶体增殖物激活受体-γ共激活因子-1α(PGC-1α)。因此,Yo-yo节食的做法通过调节线粒体动力学和相关的生物信号通路来延长果蝇的寿命。
