PDF(Pubmed)
Abstract：
While RNAs are soluble in vitro, their solubility may be altered when incorporated into some protein complexes inside the cell. The solubility phase transition of RNAs is thus indicative of changes in the function and activity of RNAs. Here, we present a protocol for the assessment of RNA solubility phase transition during Xenopus oocyte maturation. We describe steps for sample preparation, cell fractionation, RNA extraction, real-time PCR, and analysis of the obtained results. For complete details on the use and execution of this protocol, please refer to Hwang et al. (2023).1.
摘要：
虽然RNA在体外是可溶的，当掺入细胞内的一些蛋白质复合物时，它们的溶解度可能会改变。因此,RNA的溶解度相变指示RNA的功能和活性的变化。这里,我们提出了一种评估非洲爪的卵母细胞成熟过程中RNA溶解度相变的方案.我们描述了样品制备的步骤，细胞分级分离，RNA提取,实时PCR，并对所得结果进行分析。有关此协议的使用和执行的完整详细信息，请参考Hwang等人。（2023）.1。
