Abstract：
This study examined the effects of ergothioneine (EGT) supplementation as an antioxidant on the quality of boar spermatozoa when using liquid and frozen preservation methods. In the first experiment, boar semen was preserved in an extender supplemented with 0, 50, 100 and 200 µM EGT, at 15 °C, part of the samples for one and another part for three weeks. In comparison with the control (without EGT), EGT supplementation at 100 µM significantly increased the percentage of total motility of spermatozoa that were preserved as a liquid both for one and three weeks (P < 0.05). EGT supplementation did not affect the quality of preserved spermatozoa, irrespective of the EGT concentration. In the second experiment, semen was frozen and thawed in the freezing extender supplemented with 0, 50, 100 and 200 µM EGT. In comparison with the control, the 100 µM EGT supplementation significantly increased the percentages of total and progressive motility of frozen-thawed spermatozoa (P < 0.05). EGT (100 µM) supplementation did not affect the viability, the plasma membrane integrity, or the acrosomal integrity of frozen-thawed spermatozoa. These findings indicate that supplementing extenders with 100 µM EGT may improve the motility of boar sperm in both liquid and freezing preservation methods.
摘要：
这项研究研究了使用液体和冷冻保存方法时，补充麦角硫因（EGT）作为抗氧化剂对猪精子质量的影响。在第一个实验中,公猪精液保存在补充0、50、100和200μMEGT的延伸器中，在15°C,样品的一部分和另一部分三个星期。与对照（无EGT）相比，100µM的EGT补充显着增加了精子的总运动百分比，这些精子以液体形式保存了一周和三周（P<0.05）。补充EGT不会影响保存精子的质量，与EGT浓度无关。在第二个实验中,精液在补充有0、50、100和200µMEGT的冷冻补充剂中冷冻和解冻。与对照相比，补充100µMEGT可显著增加冻融精子的总运动性和进行性运动性百分比(P<0.05).EGT（100µM）补充不影响生存力，质膜的完整性，或冻融精子的顶体完整性。这些发现表明，在液体和冷冻保存方法中，补充100µMEGT的增量剂可能会提高公猪精子的运动性。
