PDF(Pubmed)
Abstract：
Conjugation plays an important role in the dissemination of antimicrobial resistance genes. Besides, this process is influenced by many biotic and abiotic factors, especially temperature. This study aimed to investigate the effect of different conditions of temperature and storage (time and recipient) of poultry meat, intended for the final consumer, affect the plasmid transfer between pathogenic (harboring the IncB/O-plasmid) and non-pathogenic Escherichia coli organisms. The determination of minimal inhibitory concentrations (MIC) of ampicillin, cephalexin, cefotaxime, and ceftazidime was performed before and after the conjugation assay. It was possible to recover transconjugants in the poultry meat at all the treatments, also these bacteria showed a significant increase of the MIC for all antimicrobials tested. Our results show that a non-pathogenic E. coli can acquire an IncB/O-plasmid through a conjugation process in poultry meat, even stored at low temperatures. Once acquired, the resistance genes endanger public health especially when it is about critically and highly important antimicrobials to human medicine.
摘要：
共轭在抗菌药物耐药基因的传播中起着重要作用。此外,这个过程受到许多生物和非生物因素的影响，尤其是温度。本研究旨在探讨禽肉的温度和储存条件（时间和接受者）的影响，面向最终消费者，影响致病性（携带IncB/O质粒）和非致病性大肠杆菌之间的质粒转移。氨苄青霉素最低抑菌浓度(MIC)的测定,头孢氨苄,头孢噻肟,和头孢他啶在缀合测定之前和之后进行。在所有的处理中都有可能恢复禽肉中的转运蛋白，对于所有测试的抗微生物剂,这些细菌也显示出MIC的显著增加。我们的结果表明，非致病性大肠杆菌可以通过结合过程在禽肉中获得IncB/O质粒，甚至储存在低温下。一旦获得，抗性基因危害公众健康，特别是当它是关于关键和非常重要的抗菌药物对人类医学。
