Abstract：
Enterovirus G (EV-G) has recently been shown to affect weight gain and cause neurological symptoms in piglets. However, the serological investigation of EV-G is limited. In this study, we developed a novel serological detection method based on the structural protein, VP1 of EV-G. The intra-assay and inter-assay coefficient variations were 3.2-8.9% and 2.6-8.0%, respectively. There was no cross-reaction of the VP1-based enzyme-linked immunosorbent assay (ELISA) with antisera against the other known porcine viruses. In addition, a comparison was made with other methods including the developed indirect ELISAs based on VP2 and VP3 proteins and western blot (WB) analysis, which demonstrated the reliability of the novel method. Using the VP1-based ELISA, we carried out the first seroepidemiological survey of EV-G in China by testing 1041 serum samples collected from different pig farms in Guangxi from 2019 to 2021. Our results showed that 68.78% of the serum samples and 100% of the pig farms were positive for EV-G, with a relatively high incidence of seropositivity in pigs of different ages. This was specifically evident in fattening pigs and sows, which may suggest that the piglets have experienced an infection with EV-G during their growth process. Our data provide the first serological evidence of EV-G infections in pigs from China and reveal the widespread presence of EV-G infections in Guangxi, China.
摘要：
肠道病毒G（EV-G）最近已被证明会影响仔猪的体重增加并引起神经系统症状。然而,EV-G的血清学研究是有限的。在这项研究中,我们开发了一种新的基于结构蛋白的血清学检测方法，EV-G的VP1测定内和测定间系数变化分别为3.2~8.9%和2.6~8.0%,分别。基于VP1的酶联免疫吸附测定（ELISA）与针对其他已知猪病毒的抗血清没有交叉反应。此外,与其他方法进行了比较，包括基于VP2和VP3蛋白的间接ELISA和蛋白质印迹（WB）分析，这证明了新方法的可靠性。使用基于VP1的ELISA，我们对2019-2021年广西不同猪场采集的1,041份血清样本进行了首次EV-G血清流行病学调查。结果显示68.78%的血清样本和100%的猪场均为EV-G阳性,在不同年龄的猪中血清阳性发生率相对较高。这在育肥猪和母猪中尤为明显，这可能表明仔猪在生长过程中经历了EV-G的感染。我们的数据提供了中国猪EV-G感染的第一个血清学证据，并揭示了EV-G感染在广西的广泛存在，中国。
