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Abstract：
Globularia alypum L. (GA) belonging to the Globulariaceae family is a Mediterranean plant which is widely used in traditional Tunisian medicine. The aim of this study was to investigate the phytochemical composition, antioxidant, anti-arthritic, antiproliferative, antibacterial and antibiofilm potential of aqueous GA leaf extracts (AGAL). Quantitative analyses of the different constituents of extracts were evaluated by high-performance liquid chromatography with photodiode-array detection (HPLC-DAD). Spectrophotometric methods and chemical tests were used for antioxidant and anti-arthritic activities. The antiproliferative study was evaluated using colorectal cancer SW620 cells, while the antibacterial assessment and analysis of the antibiofilm effects were determined by the microdilution method and the crystal violet assay, respectively. AGAL extracts presented several components, mainly Nepetin-7-Glucoside and trans-ferrulic acid. The results showed that they had an important antioxidant (IC50 = 0.34; 0.38 and 1.20 mg/mL) and anti-arthritic (IC50 = 2.94 mg/mL) properties, and these effects are displayed in a dose-dependent manner. In addition, this extract demonstrated significant antiproliferative (IC50 = 50 µg/mL), antibacterial (MIC = 6.25 mg/mL and MBC = 6.25 mg/mL), and antibiofilm (59.70% at 25 mg/mL) properties especially against S. aureus. The results achieved confirm the important role of this plant as a source of therapeutic activities.
摘要：
属于globulariaceae家族的globulariaalypumL.（GA）是一种地中海植物，广泛用于突尼斯传统医学。这项研究的目的是调查植物化学成分，抗氧化剂，抗关节炎,抗增殖,水性GA叶提取物（AGAL）的抗菌和抗生物膜潜力。通过具有光电二极管阵列检测（HPLC-DAD）的高效液相色谱法评估提取物不同成分的定量分析。分光光度法和化学测试用于抗氧化和抗关节炎活性。使用结直肠癌SW620细胞评估抗增殖研究，同时通过微量稀释法和结晶紫测定法测定抗生物膜效果的抗菌评估和分析，分别。AGAL提取物呈现了几个成分，主要是Nepetin-7-葡萄糖苷和反式铁酸。结果表明，它们具有重要的抗氧化剂（IC50=0.34；0.38和1.20mg/mL）和抗关节炎（IC50=2.94mg/mL）特性，这些效应以剂量依赖性方式显示。此外,该提取物显示出显着的抗增殖（IC50=50µg/mL），抗菌（MIC=6.25mg/mL，MBC=6.25mg/mL），和抗生物膜（25mg/mL时为59.70％）特性，尤其是针对金黄色葡萄球菌。获得的结果证实了该植物作为治疗活性来源的重要作用。
