Abstract：
High-quality genomic DNA extraction is fundamental for the study of gene cloning and expression in plants. Therefore, this study evaluated several methods for extracting genomic DNA from shoots of four Dendrocalamus species to determine the optimal technique. Genomic DNA was extracted using three different methods: a commercial DNA extraction kit method, a modified cetyltrimethylammonium bromide method and a sodium dodecyl sulfate method. A membership function analysis was employed to compare these methods. The results demonstrated that the commercial DNA extraction kit method was the most effective and comprehensive approach for extracting genomic DNA from shoots of four Dendrocalamus species. Furthermore, this study provided valuable insights into optimizing techniques for extracting genomic DNA in other bamboo species.
摘要：
高质量的基因组DNA提取是研究植物基因克隆和表达的基础。因此,这项研究评估了从四种Dendrocalamus物种的芽中提取基因组DNA的几种方法，以确定最佳技术。使用三种不同的方法提取基因组DNA：商业DNA提取试剂盒法，改进的十六烷基三甲基溴化铵法和十二烷基硫酸钠法。使用隶属函数分析来比较这些方法。结果表明，商业DNA提取试剂盒法是从4种石竹芽中提取基因组DNA的最有效和最全面的方法。此外，这项研究为优化其他竹种基因组DNA提取技术提供了有价值的见解。
