Abstract：
OBJECTIVE: To evaluate the correlation among the three molecular typing method of pulsed field gel electrophoresis(PFGE), repetitive extragenic palindromic(REP)-PCR and en-terobacterial repetitive intergenic consensus(ERIC)-PCR, and to explore the genetic relationship among strains, and to further understand the distribution and epidemic trend of Vibrio parahaemolyticus in Liaoning Province by combining Serotype analysis.
METHODS: Serum typing, PFGE, REP-PCR, and ERIC-PCR molecular typing and cluster analysis were performed on 150 VP isolates from Liaoning Province in 2018.
RESULTS: 118 isolates could be divided into 14 Serotype, and 32 isolates could not be classified. The main serotypes were O3, O1 and O2. The resolution(DI) of PFGE is 0.969, the resolution(DI) of REP-PCR is 0.948, and the resolution(DI) of ERIC-PCR is 0.927. The Serotype O3 group strains are highly similar to the molecular types of O1 group strains.
CONCLUSIONS: In 2018, the epidemic Serotype of clinical VP isolates in Liaoning Province is still O3: K6, and the epidemic serotype of food VP isolates is still O2. The result of PFGE, REP-PCR, and ERIC-PCR typing method are consistent, and the resolution and reproducibility of PFGE typing method are superior to the other two method. The Serotype O3 group is closely related to O1 group.
METHODS: Serum typing, PFGE, REP-PCR, and ERIC-PCR molecular typing and cluster analysis were performed on 150 VP isolates from Liaoning Province in 2018.
RESULTS: 118 isolates could be divided into 14 Serotype, and 32 isolates could not be classified. The main serotypes were O3, O1 and O2. The resolution(DI) of PFGE is 0.969, the resolution(DI) of REP-PCR is 0.948, and the resolution(DI) of ERIC-PCR is 0.927. The Serotype O3 group strains are highly similar to the molecular types of O1 group strains.
CONCLUSIONS: In 2018, the epidemic Serotype of clinical VP isolates in Liaoning Province is still O3: K6, and the epidemic serotype of food VP isolates is still O2. The result of PFGE, REP-PCR, and ERIC-PCR typing method are consistent, and the resolution and reproducibility of PFGE typing method are superior to the other two method. The Serotype O3 group is closely related to O1 group.
摘要：
目的:评价脉冲场凝胶电泳(PFGE)三种分子分型方法的相关性,重复基因外回文（REP）-PCR和超细菌重复基因间共识（ERIC）-PCR，并探索菌株之间的遗传关系，结合血清型分析,进一步了解辽宁省副溶血性弧菌的分布和流行趋势。
方法：血清分型，PFGE,REP-PCR,对2018年辽宁省150株VP分离株进行ERIC-PCR分子分型和聚类分析。
结果：118个分离株可分为14个血清型，32个分离株无法分类。主要血清型为O3、O1和O2。PFGE的分辨率(DI)为0.969,REP-PCR的分辨率(DI)为0.948,ERIC-PCR的分辨率(DI)为0.927。血清型O3组菌株与O1组菌株的分子类型高度相似。
结论：2018年，辽宁省临床VP分离株流行血清型仍为O3:K6，食品VP分离株流行血清型仍为O2。PFGE的结果，REP-PCR,ERIC-PCR分型方法是一致的，PFGE分型法的分辩率和重现性均优于其他两种分型法。血清型O3组与O1组密切相关。
方法：血清分型，PFGE,REP-PCR,对2018年辽宁省150株VP分离株进行ERIC-PCR分子分型和聚类分析。
结果：118个分离株可分为14个血清型，32个分离株无法分类。主要血清型为O3、O1和O2。PFGE的分辨率(DI)为0.969,REP-PCR的分辨率(DI)为0.948,ERIC-PCR的分辨率(DI)为0.927。血清型O3组菌株与O1组菌株的分子类型高度相似。
结论：2018年，辽宁省临床VP分离株流行血清型仍为O3:K6，食品VP分离株流行血清型仍为O2。PFGE的结果，REP-PCR,ERIC-PCR分型方法是一致的，PFGE分型法的分辩率和重现性均优于其他两种分型法。血清型O3组与O1组密切相关。
