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Abstract：
Oral squamous cell carcinoma (OSCC) is one of the most common malignant tumours with increasing incidence, and oral leukoplakia (OLK) has a strong tendency to undergo malignant transformation. The oral microbiota may influence oral cancer progression, but the salivary bacterial composition and functional changes in OSCC and OLK have not been comprehensively elucidated. Therefore, we compared salivary bacteria in OLK and OSCC patients with healthy controls (HC).
Metagenomic sequencing was used to compare the bacterial composition and functional changes of 18 OSCC patients, 21 OLK patients and 21 HC. Spearman correlation was used to identify possible associations between functions and bacteria.
Gemella was the most differentially enriched genus in OSCC. At the species level, Streptococcus sp. NPS 308, Streptococcus agalactiae, Gemella haemolysans and Gemella morbillorum were slightly increased in OLK and OSCC. Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that OSCC was mainly associated with metabolism functions, including lipid metabolism, carbohydrate metabolism and glycan biosynthesis and metabolism. The synthesis and degradation of ketone bodies, cysteine and methionine metabolism and glycerolipid metabolism differed significantly among the three groups, and were highest in OSCC and lowest in HC. And G. haemolysans was significantly associated with these selected metabolic pathways.
Metagenomic analysis revealed significant differences in the salivary microbiota among OSCC, OLK and HC. Thus, salivary microbiota composition and functional changes may be associated with OSCC progression. Metabolism of nonessential amino acids such as cysteine and methionine in bacteria may play an important role in oral oncogenesis, and more studies of the mechanism between metabolisms of bacteria and oral oncogenesis are needed in the future.
Metagenomic sequencing was used to compare the bacterial composition and functional changes of 18 OSCC patients, 21 OLK patients and 21 HC. Spearman correlation was used to identify possible associations between functions and bacteria.
Gemella was the most differentially enriched genus in OSCC. At the species level, Streptococcus sp. NPS 308, Streptococcus agalactiae, Gemella haemolysans and Gemella morbillorum were slightly increased in OLK and OSCC. Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that OSCC was mainly associated with metabolism functions, including lipid metabolism, carbohydrate metabolism and glycan biosynthesis and metabolism. The synthesis and degradation of ketone bodies, cysteine and methionine metabolism and glycerolipid metabolism differed significantly among the three groups, and were highest in OSCC and lowest in HC. And G. haemolysans was significantly associated with these selected metabolic pathways.
Metagenomic analysis revealed significant differences in the salivary microbiota among OSCC, OLK and HC. Thus, salivary microbiota composition and functional changes may be associated with OSCC progression. Metabolism of nonessential amino acids such as cysteine and methionine in bacteria may play an important role in oral oncogenesis, and more studies of the mechanism between metabolisms of bacteria and oral oncogenesis are needed in the future.
摘要：
背景：口腔鳞状细胞癌（OSCC）是最常见的恶性肿瘤之一，发病率越来越高，口腔白斑(OLK)有强烈的恶变倾向。口腔微生物群可能会影响口腔癌的进展，但是OSCC和OLK的唾液细菌组成和功能变化尚未得到全面阐明。因此,我们将OLK和OSCC患者的唾液细菌与健康对照(HC)进行了比较.
方法：采用宏基因组测序法比较18例OSCC患者的细菌组成和功能变化，21名OLK患者和21名HC。Spearman相关性用于鉴定功能与细菌之间可能的关联。
结果：Gemella是OSCC中差异最丰富的属。在物种层面,链球菌。NPS308无乳链球菌,OLK和OSCC中的血溶菌和memellamorbillorum略有增加。京都基因和基因组百科全书(KEGG)结果显示OSCC主要与代谢功能有关,包括脂质代谢，碳水化合物代谢和聚糖生物合成和代谢。酮体的合成与降解,半胱氨酸和蛋氨酸代谢和甘油脂代谢在三组之间差异显著，OSCC最高，HC最低。并且G.hemolysans与这些选定的代谢途径显着相关。
结论：宏基因组分析显示，OSCC之间的唾液菌群存在显着差异，OLK和HC。因此，唾液微生物组成和功能变化可能与OSCC进展相关.细菌中半胱氨酸和蛋氨酸等非必需氨基酸的代谢可能在口腔肿瘤发生中起重要作用。未来需要更多的细菌代谢与口腔癌发生之间的机制研究。
方法：采用宏基因组测序法比较18例OSCC患者的细菌组成和功能变化，21名OLK患者和21名HC。Spearman相关性用于鉴定功能与细菌之间可能的关联。
结果：Gemella是OSCC中差异最丰富的属。在物种层面,链球菌。NPS308无乳链球菌,OLK和OSCC中的血溶菌和memellamorbillorum略有增加。京都基因和基因组百科全书(KEGG)结果显示OSCC主要与代谢功能有关,包括脂质代谢，碳水化合物代谢和聚糖生物合成和代谢。酮体的合成与降解,半胱氨酸和蛋氨酸代谢和甘油脂代谢在三组之间差异显著，OSCC最高，HC最低。并且G.hemolysans与这些选定的代谢途径显着相关。
结论：宏基因组分析显示，OSCC之间的唾液菌群存在显着差异，OLK和HC。因此，唾液微生物组成和功能变化可能与OSCC进展相关.细菌中半胱氨酸和蛋氨酸等非必需氨基酸的代谢可能在口腔肿瘤发生中起重要作用。未来需要更多的细菌代谢与口腔癌发生之间的机制研究。
