PDF(Pubmed)
Abstract：
Here, we present a protocol for single-molecule super-resolution imaging of the nuclear export of pre-ribosomal subunits pre-40S and pre-60S through nuclear pore complexes. We describe steps for plating cells and co-transfecting cells. We then detail steps for using single-point edge-excitation sub-diffraction microscopy, allowing visualization of real-time dynamics of the pre-ribosomal subunits. For complete details on the use and execution of this protocol, please refer to Junod et al. (2023).1.
摘要：
这里,我们提出了通过核孔复合物对前核糖体亚基前40S和前60S的核输出进行单分子超分辨率成像的方案。我们描述了铺板细胞和共转染细胞的步骤。然后，我们详细说明使用单点边缘激发亚衍射显微镜的步骤，允许可视化核糖体前亚基的实时动态。有关此协议的使用和执行的完整详细信息，请参阅Junod等人。（2023）.1。
