{Reference Type}: Journal Article
{Title}: Protocol for live-cell super-resolution imaging of transport of pre-ribosomal subunits through the nuclear pore complex.
{Author}: Tingey M;Junod SL;Rush C;Yang W;
{Journal}: STAR Protoc
{Volume}: 5
{Issue}: 1
{Year}: 2024 Mar 15
暂无{DOI}: 10.1016/j.xpro.2023.102790
{Abstract}: Here, we present a protocol for single-molecule super-resolution imaging of the nuclear export of pre-ribosomal subunits pre-40S and pre-60S through nuclear pore complexes. We describe steps for plating cells and co-transfecting cells. We then detail steps for using single-point edge-excitation sub-diffraction microscopy, allowing visualization of real-time dynamics of the pre-ribosomal subunits. For complete details on the use and execution of this protocol, please refer to Junod et al. (2023).1.