%0 Journal Article
%T Protocol for live-cell super-resolution imaging of transport of pre-ribosomal subunits through the nuclear pore complex.
%A Tingey M
%A Junod SL
%A Rush C
%A Yang W
%J STAR Protoc
%V 5
%N 1
%D 2024 Mar 15
%M 38113144
暂无%R 10.1016/j.xpro.2023.102790
%X Here, we present a protocol for single-molecule super-resolution imaging of the nuclear export of pre-ribosomal subunits pre-40S and pre-60S through nuclear pore complexes. We describe steps for plating cells and co-transfecting cells. We then detail steps for using single-point edge-excitation sub-diffraction microscopy, allowing visualization of real-time dynamics of the pre-ribosomal subunits. For complete details on the use and execution of this protocol, please refer to Junod et al. (2023).1.