Abstract：
The current work aimed to analyze, morphologically, statistically, and molecularly, oocysts shed from plumbeous pigeons, Patagioenas plumbea (Vieillot, 1818), from a locality at 2197 m of altitude near the Agulhas Negras peak, the highest point of the State of Rio de Janeiro, southeastern Brazil. The oocysts were extremely polymorphic, being subspheroidal, ovoidal, or ellipsoidal, in addition to having the random presence/absence of characteristic features associated with the oocyst wall, such as micropyle, micropyle cap, lateral micropyle, and outer veil/rough wall. Linear regression confirmed the extreme polymorphism of oocysts, showing that if all combinations of taxonomic characters in oocysts (morphotypes) were overestimated, 19 different species could be identified/described. In contrast, the means comparison analysis between oocysts with the presence/absence of characteristic features and the histograms showed equivalences and regularity in the distribution in the classes of measures, which indicate the presence of a single species in the measured oocysts. Molecular analyses were performed from the isolation of individual oocysts of different morphotypes, which had their genetic material extracted, amplified, and sequenced in 4 non-overlapping loci in the cox1 and cox3 genes and fragments of the small and large subunit rDNA of mitochondrial DNA. The sequences were 100% identical between the morphotypes, with the exception of a very small divergence observed at the locus that partially covers the cox3 gene. The phylogenetic analysis was inconclusive for the locus within the cox1 gene traditionally used for eimeriid coccidians; however, the other loci should have a promising future for phylogenetic studies when more sequences for the same genic regions are deposited in GenBank. Finally, the multifactorial analysis of the current work supported that the polymorphic oocysts shed from P. plumbea are a single species, which was named Eimeria patagioenasae, making this the twenty-second eimerian description from Columbiformes.
摘要：
目前的工作旨在分析，形态学上,统计，在分子上，卵囊从羽绒服的鸽子身上脱落,Patagioenasplumbea(Vieillot，1818),从阿古拉斯内格拉斯峰附近海拔2197米的地方出发，里约热内卢州的最高点,巴西东南部。卵囊是极其多态的，是亚球形的，卵形,或椭圆体，除了具有与卵囊壁相关的特征的随机存在/不存在，比如微管，微孔帽，外侧小喷管，和外部面纱/粗糙的墙壁。线性回归证实了卵囊的极端多态性，表明如果卵囊（形态型）中所有分类特征的组合都被高估了，可以鉴定/描述19种不同的物种。相比之下,存在/不存在特征性特征的卵囊与直方图之间的均值比较分析显示了度量类别中分布的等效性和规律性，这表明在测量的卵囊中存在单个物种。从不同形态类型的单个卵囊的分离中进行分子分析。提取了他们的遗传物质，放大，并测序了cox1和cox3基因中的4个非重叠基因座以及线粒体DNA的小亚基和大亚基rDNA的片段。形态类型之间的序列是100%相同的，除了在部分覆盖cox3基因的基因座上观察到的非常小的差异。对于cox1基因内的基因座，系统发育分析尚无定论传统上用于Eimeriid球虫；但是，当相同基因区域的更多序列存放在GenBank中时，其他基因座对于系统发育研究应该有一个有希望的未来。最后,对当前工作的多因素分析支持从P.plumbea脱落的多态卵囊是单一物种，它被命名为艾美耳球虫，使这成为Columbiformes的第二十二次Eimerian描述。
