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Abstract：
Cajal-Retzius (CR) cells are a transient neuron type that populate the postnatal hippocampus. To understand how the persistence of CR cells influences the maturation of hippocampal circuits, we combined a specific transgenic mouse line with viral vector injection to selectively ablate CR cells from the postnatal hippocampus. We observed layer-specific changes in the dendritic complexity and spine density of CA1 pyramidal cells. In addition, transcriptomic analysis highlighted significant changes in the expression of synapse-related genes across development. Finally, we were able to identify significant changes in the expression levels of latrophilin 2, a postsynaptic guidance molecule known for its role in the entorhinal-hippocampal connectivity. These findings were supported by changes in the synaptic proteomic content in CA1 stratum lacunosum-moleculare. Our results reveal a crucial role for CR cells in the establishment of the hippocampal network.
摘要：
Cajal-Retzius（CR）细胞是出生后海马的瞬时神经元类型。为了了解CR细胞的持久性如何影响海马回路的成熟，我们将特定的转基因小鼠系与病毒载体注射相结合，以选择性地从出生后的海马中消融CR细胞。我们观察到CA1锥体细胞的树突复杂性和脊柱密度的层特异性变化。此外,转录组学分析强调了突触相关基因在整个发育过程中的表达的显着变化。最后,我们能够确定latrophilin2表达水平的显著变化,latrophilin2是一种突触后指导分子,以内嗅-海马连接的作用而闻名.这些发现得到了CA1腔隙分子中突触蛋白质组含量变化的支持。我们的结果揭示了CR细胞在海马网络建立中的关键作用。
